(sqk-lsk109) . below are the results regarding the n50 variation. we found no significant difference in throughput (all samples generated 3 star performance; 8+ gb on flo-min106). we recommend customers are mindful of the effects of different storage conditions of cells and advice to store ...
为连接测序试剂盒(SQK-LSK109 和 SQK-LSK110)进行了组分体积的优化; 更简化的订购和库存管理; 适用于所有 Nanopore 测序仪:Minion®、Gridion®、Promethion®、Floungle®; 杜绝浪费 - 没有多余的缓冲液或过量的试剂。 特别提醒: 使用这些试剂时,请遵循 Oxford Nanopore Technologies 相应设备连接测序试剂盒...
• PCR barcoding amplicons (with SQK-LSK109) • PCR barcoding genomic DNA (with SQK-LSK110) • PCR barcoding amplicons (with SQK-LSK110) To access the protocols, you will need to register for a Nanopore Community account. Safety and legal Safety Data Sheet for PCR Barcoding Expansion...
DNA was eluted in TE buffer, aliquoted and stored at 4°C or -20°C. After set periods of time over twelve months, aliquots are removed from storage and libraries prepared for sequencing using theLigation Sequencing Kit (SQK-LSK109). Prior to sequencing, the samples wereSPRI size-selected....
The for 30 minutes resulting DNA ownasaqHuuanlatimfieixdeurs; i6n1g µtlhoefQthuebietluHaSteDwNaAs tkhiet.n transferred into a clean 1.5 ml Adapter ligation and clean-up was performed using the Ligation Sequencing Kit SQK-LSK109 (Oxford Nanopore Technologies, Oxford, United Kingdom)...
这是目前最为全面系统地采用牛津纳米孔测序技术进行 mRNA疫苗质量分析的研究,鉴于 mRNA 疫苗和疗法的巨大优势,Oxford Nanopore 公司正在大力宣传这些研究成果,以期在竞争日益激烈的基因测序领域寻求优势。 VAX-seq 在 mRNA 疫苗制备工艺各个阶段的分析大致可分为三部分:第一,采用 LSK109 连接测序试剂盒,构建质粒 DNA ...
这是目前最为全面系统地采用牛津纳米孔测序技术进行 mRNA疫苗质量分析的研究,鉴于 mRNA 疫苗和疗法的巨大优势,Oxford Nanopore 公司正在大力宣传这些研究成果,以期在竞争日益激烈的基因测序领域寻求优势。 VAX-seq 在 mRNA 疫苗制备工艺各个阶段的分析大致可分为三部分:第一,采用LSK109 连接测序试剂盒,构建质粒 DNA ...
Oxford Nanopore DNA sequencing Library preparation was carried out using the ligation sequencing kits (Oxford Nanopore Technologies) SQK-LSK109 and SQK-LSK112 for sequencing on R.9.4.1 and the R.10.4 flowcells, respectively. Anaerobic digester and Zymo R.9.4.1 datasets were generated on a MinION...
所使用的建库试剂如下:NEBNext ARTIC SARS-CoV-2 配套试剂盒(Oxford Nanopore Technologies)、Oxford Nanopore Technologies Native Barcoding Expansion kits 1-12(EXP-NBD104)、13-24 (EXP-NBD114)、Ligation Sequencing Kit(EXP-NBD114)、Ligation Sequencing Kit(SQK-LSK109)和 SFB Expansion Kit(EXP-SFB001)。
ONT libraries were prepared according to the following protocol, using the Oxford Nanopore SQK-LSK109 kit. Genomic DNA or DNA previously fragmented to 50 kb with a Megaruptor (Diagenode S.A., Liege, Belgium) was first size-selected using a BluePippin (Sage Science, Beverly, MA, USA). Th...