(sqk-lsk109) . below are the results regarding the n50 variation. we found no significant difference in throughput (all samples generated 3 star performance; 8+ gb on flo-min106). we recommend customers are mindful of the effects of different storage conditions of cells and advice to store ...
现在您可更方便地通过同一个货号 E7180 订购以上所有模块,并且该产品已为 Oxford Nanopore Technologies 连接测序试剂盒(SQK-LSK109 和 SQK-LSK110)优化了体积。此次,我们又推出了 96 次反应的 L 包装,再次助力您便捷完成 ONT 实验。 产品特点 为连接测序试剂盒(SQK-LSK109 和 SQK-LSK110)进行了组分体积的优化...
DNA was eluted in TE buffer, aliquoted and stored at 4°C or -20°C. After set periods of time over twelve months, aliquots are removed from storage and libraries prepared for sequencing using theLigation Sequencing Kit (SQK-LSK109). Prior to sequencing, the samples wereSPRI size-selected....
The for 30 minutes resulting DNA ownasaqHuuanlatimfieixdeurs; i6n1g µtlhoefQthuebietluHaSteDwNaAs tkhiet.n transferred into a clean 1.5 ml Adapter ligation and clean-up was performed using the Ligation Sequencing Kit SQK-LSK109 (Oxford Nanopore Technologies, Oxford, United Kingdom)...
• PCR barcoding genomic DNA (with SQK-LSK109) • PCR barcoding amplicons (with SQK-LSK109) • PCR barcoding genomic DNA (with SQK-LSK110) • PCR barcoding amplicons (with SQK-LSK110) To access the protocols, you will need to register for a Nanopore Community account. Safety and...
VAX-seq 在 mRNA 疫苗制备工艺各个阶段的分析大致可分为三部分:第一,采用LSK109 连接测序试剂盒,构建质粒 DNA 测序文库,对质粒序列、长度、纯度进行分析;第二,采用SQK-PCS111试剂盒,以 mRNA 作为模板,构建cDNA 测序文库,对 mRNA 序列、长度、完整性、纯度、PolyA 做出分析;第三,采用SQK-RNA002,进行直接 RNA ...
VAX-seq 在 mRNA 疫苗制备工艺各个阶段的分析大致可分为三部分:第一,采用 LSK109 连接测序试剂盒,构建质粒 DNA 测序文库,对质粒序列、长度、纯度进行分析;第二,采用SQK-PCS111 试剂盒,以 mRNA 作为模板,构建cDNA 测序文库,对 mRNA 序列、长度、完整性、纯度、PolyA 做出分析;第三,采用 SQK-RNA002,进行直接 ...
Library preparation was carried out using the ligation sequencing kits (Oxford Nanopore Technologies) SQK-LSK109 and SQK-LSK112 for sequencing on R.9.4.1 and the R.10.4 flowcells, respectively. Anaerobic digester and Zymo R.9.4.1 datasets were generated on a MinION Mk1B (Oxford Nanopore Techno...
所使用的建库试剂如下:NEBNext ARTIC SARS-CoV-2 配套试剂盒(Oxford Nanopore Technologies)、Oxford Nanopore Technologies Native Barcoding Expansion kits 1-12(EXP-NBD104)、13-24 (EXP-NBD114)、Ligation Sequencing Kit(EXP-NBD114)、Ligation Sequencing Kit(SQK-LSK109)和 SFB Expansion Kit(EXP-SFB001)。
ONT libraries were prepared according to the following protocol, using the Oxford Nanopore SQK-LSK109 kit. Genomic DNA or DNA previously fragmented to 50 kb with a Megaruptor (Diagenode S.A., Liege, Belgium) was first size-selected using a BluePippin (Sage Science, Beverly, MA, USA). Th...