Fully characterise human genomic, epigenomic, and transcriptomic variation with real-time nanopore sequencing. Resolve complex regions, uncover previously hidden variation, and identify base modifications as standard with any-length reads. Generate highl
Fully characterise human genomic, epigenomic, and transcriptomic variation with real-time nanopore sequencing. Resolve complex regions, uncover previously hidden variation, and identify base modifications as standard with any-length reads. Generate highl
The assembly with the long nanopore reads was able to correctly assemble gene cassettes, rRNAs, transposable elements, and other genomic features that were almost entirely absent in an assembly using Illumina sequencing alone (with a contig N50 of only 59,927bp)....
Nanopore-based assemblies consisted of Minimap2 v. 2.17 (ref.33), Racon v. 1.3.3 (used three times)34, Medaka v. 1.4.4 (used twice,https://github.com/nanoporetech/medaka), and one round of Racon with Illumina reads. For the short-read assembly the trimmed Illumina reads were ...
team in Canada successfully developed a rapid workflow using nanopore sequencing for HLA typing in the context of clinical transplantation. Sequencing was performed between 6h and 12h, although sufficient reads for HLA assignment were achieved within 1 hr. Read more...
Choosing the optimum assembly approach is essential to achieving a high-quality genome assembly suitable for comparative and evolutionary genomic investigations. Significant recent progress in long-read sequencing technologies such as PacBio and Oxford Nanopore Technologies (ONT) has also brought about a ...
ocellaris genome will be an invaluable molecular resource for supporting a range of genetic, genomic, and phylogenetic studies specifically for clownfish and more generally for other related fish species of the family Pomacentridae. 展开 关键词: clownfish long reads genome transcriptome hybrid assembly ...
Oxford Nanopore Sequencers. Image Source: Oxford Nanopore Technologies (ONT). The nanopores only allow the passage of single-stranded DNA in a linear sequence. This method provides real-time analysis and can sequence nucleotides from short to ultra-long reads. ...
At the annual London Calling conference, CTO Clive Brown and colleagues James Clarke, Stuart Reid, Lakmal Jayasinghe and Rosemary Sinclair Dokos have provided an Oxford Nanopore tech update summarising the status of its nanopore sensing platform, key rec
bacpaq is a bioinformatics best-practice pipeline for bacterial genomic analysis for short-reads (Illumina) and long-reads (Oxford Nanopore) sequencing data. Currently bacpaq supports WGS-based analyses, however, we plan to integrate Microbiome (Amplicon and Shotgun Metagenomics) analyses in future....