Adaptive sampling enables selection of individual DNA molecules from sequencing libraries, a unique property of nanopore sequencing. Here we develop our adaptive sampling tool readfish to become "barcode-aware" enabling selection of different targets within barcoded samples or filtering out individual ...
Sequence full-length fusion transcripts with nanopore reads of unrestricted length. Utilise simple, flexible workflows — with or without PCR — using cDNA or direct RNA nanopore sequencing. Rapidly identify fusions ...
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如果是原始的fast5数据,需要通过使用Dorado(或GUPPY)软件将电信号转化为碱基序列。一般来说,测序服务商会把拆分好barcode的.bam或/和.fastq文件给到用户,不需要用户自己做转换。 2. 原始下机数据质量统计 -- NanoComp 关于ONT下机数据的质量统计和可视化,可参考前面的详细教程:三代测序 - Oxford Nanopore (ONT) ...
used alternative is Oxford Nanopore sequencing, which offers low-cost long-read data. However, numerous studies have shown that despite vast improvements in raw error rates, assembly consensus sequences still contain insertions and deletions in homopolymers (indels) that often cause frameshift errors ...
临床输血与检验 2024年6月第26卷第3期 ·365· ·论著· Oxford Nanopore Technology在HLA-DPB1 模棱两可基因分型中的 应用初探 廉雪 张丹 温玉洁 范成艳 王东梅 刘娜 张荣芳 贾延军 北京市红十字血液中心,北京 100088 DOI:10.3969/j.issn.1671-2587.2024.03.011 作者简介:廉雪,主要从事HLA分型及血小板抗体研究...
2.4. ONT sequence analysis and assembly Qcat (Oxford Nanopore Technology), python command-line tool for demultiplexing ONT reads from FASTQ files, was used to trim adaptor and barcode sequences. With genomeSize = 2k and default parameters, trimmed raw reads were analysis using Canu v1.9 [42] ...
NanoGO-{Abrv}_barcode{##}_{run_id}_{dorado_models_hex}.fastqNanoGO Abbreviation ID (NanoGO-{Abrv}): This identifier is a short form for the specific stage or type of data being processed. For instance, RNR for Raw Nanopore Reads, ADTR for Adapter Trimmed Reads, etc. Each ...
In addition to supporting the standard barcode kits from Oxford Nanopore, Dorado also supports specifying custom barcode kit arrangements and sequences. This is done by passing a barcode arrangement file via the --barcode-arrangement argument (either to dorado demux or dorado basecaller). Custom ...
MinION, a third-generation sequencer from Oxford Nanopore Technologies, is a portable device that can provide long-nucleotide read data in real-time. It primarily aims to deduce the makeup of nucleotide sequences from the ionic current signals generated when passing DNA/RNA fragments through ...