After 72 h, the fluorescence intensity was observed under a fluorescence microscope, and the visible fluorescence of the cells indicated that the transfection was successful. The lentivirus was resistant to puromycin, and the stable expression lentiviral cell lines were screened by adding puromycin in ...
Immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) methods were utilized to evaluate ER, PR, and HER2 expression status according to the definitions provided by the ASCO/CAP17. ER and PR status was based on tumor nuclei staining, with a positivity threshold of ≥ 1%....
Full size image We next asked whether Treg frequency may be used to discriminate between patients with CRC and healthy subjects. ROC analyses of all Treg subsets revealed that CD45RO+CD30+OX40+, CD45RO+CD30−OX40+, and CD45RO+OX40+Tregs could clearly discriminate between CRC patients an...
A FACScan flow cytometer (Becton Dickinson) was used to measure the fluorescence intensity. With the same process, the fluorescence intensity was measured in another sample containing 500 ml of myocardial cell suspension. Intracellular free Ca2+ concentration was calculated as mean fluorescence intensity...
The [6 6 0] reflection of the three Ge(1 1 0) spherically bent crystal analyzers (bending radius of 1 m) from the multi-crystal spectrometer installed at BM16 were used to select the Lα1 (L3-M5) fluorescence line at 9442 eV. The total energy resolution of the HERFD-...
3 The cell of origin in MDS is thought to reside within the CD34+ population, based on multiparameter flow cytometry and fluorescence in situ hybridization (FISH) studies, global gene expression analysis, as well as on xenotransplantation of marrow cells from MDS patients into immunodeficient mice...
However, compared with the non-FU-CT-26 cells, the expression level and average fluorescence intensity of MHC class II molecules and of the co-stimulatory molecules CD80 and CD86 on the surface of FU-CT-26 cells were all clearly upregulated (Table 3), although the expression level of MHC ...
Expression data were reported as percentage of CD5+CD19+ CLL cells displaying specific fluorescence intensity greater than the 98% to 99% of the same cell population stained with isotype– and fluorochrome–matched control immunoglobulins. Flow cytometry detection of ZAP-70 was performed as reported...
A total of three different optical filters were used with the following characteristics: FL1 (green fluorescence; YO-PRO-1, JC-1 monomers): Dichroic/Splitter, DRLP: 550 nm, BP filter: 525 nm, detection width 505 nm–545 nm; FL2 (orange fluorescence; JC-1 aggregates): DRLP:...
which is in agreement with the XPS analysis. A slightly higher pre-edge intensity than that of Fe2O3likely indicates a distorted octahedral coordination geometry of the Fe3+sites in Ru1/D-NiFe LDH41. The R-space shows two prominent coordination peaks at 1.44 and 2.58 Å (phase uncor...