This tutorial describes how Imaris can be used to quantify chromatin modifications in 3D whole-mount plant tissue images. The approach shown here, is applicable to a wide range of plant tissue and can be used to quantify fluorescently labeled proteins targeted to the chromatin....
Possible interference of the particles with the assay was investigated by incubating the NMs with AB staining solution alone (no cells) and compared with the blank value. Relative fluorescence intensity < 10% is within the method variability, thus indicating no significant interference is present...
4C). To directly quantify the rates at which actin, CP, and the Arp2/3 complex join the growing network at the NPF-coated surface, we integrated the fluorescence intensity of each component in individual actin networks and divided these values by the reaction time (Fig. 4D). This analysis...
Alternatively, fluorescent dyes that increase their emission intensity in the presence of RNA can equally be used to quantify multiplied RNA at a microplate reader. Alternatively, fluorescence polarisation can be used to determine RdRP activity by its complementation of a template RNA strand. If ...
Predicting concentrations is crucial in natural product analysis to quantify specific compounds or groups of compounds in samples. HPLC stands out for its ability to separate, identify, and quantify mixture components. It's employed in determining ephedrine alkaloids in Ephedra products and analyzing Thi...
Simple Western requires only 3 µL of sample and offers highly sensitive multiplex detection to quantify low abundance phosphorylated and total-protein isoforms in a sample. The Stellar™ NIR/IR Modules for the Simple Western instrument Jess™ provide industry-leading fluorescence detection ...
Membrane fusion is detected as a sudden increase in DiD fluorescence intensity due to dilution of the probe into the endosomal membrane7. The time point of membrane fusion was defined as the moment when fluorescence at least doubled. DiD-labeled virions were either first pre-incubated with the...
Describe how you can quantify the size difference, between the tick and the mite, using the microscope. How can depth of field affect your ability to find and examine a specimen? Why should you move the stage away from the objectives...
The most ubiquitous radiotracer in PET/CT lung cancer imaging is 18-fluorine (18F) fluorodeoxyglucose (FDG), with several metabolic parameters used to quantify tumor aggressiveness and assess prognosis, including maximum standardized uptake value (SUVmax), metabolic tumor volume (MTV) and total lesion...
Kinetics of plasma-cell chimerism after allogeneic stem cell transplantation by highly sensitive real-time PCR based on sequence polymorphism and its value to quantify minimal residual disease in patients with multiple myeloma. Exp Hematol 2006; 34: 688–694. Article Google Scholar Galimberti S, ...