[1] Particularly for Nd 3+ , it's possible to work within the first biological window, for both the excitation and fluorescence signals. [2-5] Moreover, the spectral separation between energy levels of Nd 3+ is responsible for high thermal sensitivity (S >1% K 1 as defined below) at ...
If the fluorescence measurement is performed under transient conditions by means of pulse excitation such as with a pulsed laser or with ananosecondflash lamp, we can observe the transient behavior. Rate equation,Eq. 5, is also valid under the transient condition, whereI0can be treated as a ...
Label-free methods for imaging microtubules are preferred when long exposure times would lead to phototoxicity in fluorescence, or for retaining more native structure and activity. This method approaches quantitative phase imaging of MTs as an inverse problem based on the Transport of Intensity Equation...
The excitation wavelength for Syto9 and PI was centered at ~ 485 nm, whereas the fluorescence intensity was measured at ~ 530 nm (green) and ~ 630 nm (red), respectively. Three different locations on each specimen were scanned to get a representative image. SEM characterization of ...
The use of the ratios of intensities of spectral lines for X-ray fluorescence analysis of metal alloys and oxide materials relative intensitymetal alloysoxide materialsAn X-ray fluorescence analysis technique is proposed, which is based on using ratios of intensities of spectral ... AV Garmay,KV...
Another advantage of lifetime measurement is that as a relative measurement of the fluorescence intensity, it does not depend on a non-uniform intensity excitation of the sample, and also does not depend on molecular concentration, allowing one to detect very low quantity of molecules. Following ...
Variation of intrinsic tryptophan (Trp) fluorescence intensity and lifetime in proteins is widely exploited to follow changes in protein structure such as ... PR Callis,T Liu - 《Journal of Physical Chemistry B》 被引量: 279发表: 2004年 Quantitative Prediction of Fluorescence Quantum Yields for ...
I am currently trying to determine the curve of highest intensity from the attached image which should be the line along the centerline of the bright path. Also is it possible to create a surface of revolution from this in matlab or get the equation of the curve in 2d? A sample of the...
The fluorescence of rare-earth ions RE3+ = Pr3+, Er3+ and Tm3+ has been compared in the transition-metal fluoride glass (36mol%PbF2–24mol%ZnF2–35mol%GaF3... B Boulard,S Guy,I Vasiliev,... - 《Philosophical Magazine》 被引量: 6发表: 2004年 A comparative investigation of upconver...
We should make a clear distinction between techniques based on sputtering or laser ablation that are destructive (the analytes are removed from the sample during the analytical process and spectroscopic techniques based on fluorescence or local excitation that leave the atoms or molecules intact in the...