Previously, these organelles were shown to consist of a protein shell partly composed of the PduA protein, the majority of the cell's B[sub 12]-dependent diol dehydratase, and additional unidentified proteins. In this report, the polyhedral organelles involved in B[sub 12]-dependent 1,2-PD ...
Apart from being a site for ATP synthesis, the mitochondrion is also involved in the self-destruction of a cell in a process known as apoptosis.Ribosomes - Found in the cytoplasm and the surface of the rough endoplasmic reticulum, ribosomes are composed of RNA and proteins. They may be des...
Second, the ability of TOT to selectively detect cellular protein–DNA interactions was explored using the model POI candidates lamin A [64] and H2B [65], two proteins that are pivotal in chromatin organization and transcription regulation. To achieve this, the C-termini of these POIs were ...
The endoplasmic reticulum (ER) is another membrane system, continuous with the nuclear membrane and involved in the processing of lipids and proteins. The tubular cisternae and flattened sheets of theERcan make up about 10% of the entire cell volume. It can be classified into rough ER (associat...
Because proteins synthesis is an essential function of all cells, ribosomes are found in practically every cell. Ribosomes are particularly abundant in cells that synthesize large amounts of protein. For example, the pancreas is responsible for creating several digestive enzymes and the cells that ...
A. Domains, motifs, and scaffolds: the role of modular interactions in the evolution and wiring of cell signaling circuits. Annu. Rev. Biochem. 75, 655–680 (2006). Article CAS PubMed Google Scholar Scott, J. D. & Pawson, T. Cell signaling in space and time: where proteins come ...
This intermolecular isopeptide bond formation can be used to design stable protein complexes or to bind proteins to functionalized surfaces. To validate, whether this system could be used for organelle purification, we developed a system in which the SpyTag peptide is targeted to the surface of ...
To target the ER or Golgi network, different secreted effectors evolved to specifically hijack RAB proteins and exploit phosphoinositide lipids, which are phosphorylated derivatives of phosphatidylinositol. Mitochondria are the power plants of the cell, but are also involved in essential cellular processes...
Membranes can be stained with lectins, which are carbohydrate-binding proteins that recognize and bind to certain parts of sugars. Lectins such as wheat germ agglutinin (WGA) will stain the plasma membrane but also the Golgi, an organelle composed of many membrane sacs and vesicles involved in ...
preserved by the unfolded protein response (UPR), ER-associated degradation (ERAD), and a structured adaptive program. A variety of factors, such as altered cellular metabolism, mutations in substrate and route chaperones, and infection, are involved in predisposing proteins to misfolding [27,28]...