Analysis of genomes, transcriptomes and proteomes reveals the existence of hundreds to thousands of translated, yet non-annotated, short open reading frames (small ORFs or smORFs). The discovery of smORFs and their protein products, smORF-encoded polypep
In this study, we have established a small ORF-encoded peptides (SEPs) database from mouse GENCODE release. This database provides about half a million putative translated SEPs in mouse. We also extract serum proteins from wild type andob/obmice, and collect the low molecular weight ...
In this study, we have established a small ORF-encoded peptides (SEPs) database from mouse GENCODE release. This database provides about half a million putative translated SEPs in mouse. We also extract serum proteins from wild type andob/obmice, and collect the low molecular weight ...
d ASC spike microscopy images in GFP-ASC HeLa cells at 48 h after infection with lentiviruses carrying individual KSHV-encoded protein. Scale bar, 50 μm. e,f IL-1β production (e) and LDH release (f) in THP-1 cells infected by KSHVWT, KSHVUV, or KSHVΔORF45 for 24 h (n...
戊型肝炎病毒ORF2截短蛋白p154在毕赤酵母 中的分泌性表达及其抗原性 徐明杰,张建华,孟继鸿 (东南大学医学院,江苏南京 210009) [摘要]目的:利用毕赤酵母系统表达戊型肝炎病毒(HEV)ORF2编码蛋白第452～605氨基酸多肽(p154), 探讨毕赤酵母表达系统用于研发戊型肝炎基因工程疫苗的可能性。方法:采集戊型肝炎患者粪便...
Here we show that the proline:arginine (PR) poly-dipeptides encoded by the C9orf72 repeat expansion bound nuclear transport receptor Kapβ2 through its nuclear localization signal (NLS) recognition motif, and inhibited the ability of Kapβ2 to melt fused in sarcoma (FUS) droplets by competing...
Sequence and functional analysis of a homolog of interleukin-10 encoded by the parapoxvirus orf virus. Virus Genes 21:85-95.Fleming, S.B. et al. (2000) Sequence and functional analysis of a homolog of interleukin-10 encoded by the parapoxvirus orf virus. Virus Genes 21, 85-95...
Two complementary approaches were used in search of the intracellular targets of the toxic PR poly-dipeptide encoded by the repeat sequences expanded in the C9orf72 form of amyotrophic lateral sclerosis. The top categories of PRn-bound proteins include constituents of non-membrane invested cellular ...
was treated at 37°C for 15 minutes, however, protein aggregation was not detected. Deletion studies indicate that the presence of a hydrophobic domain downstream of the 3C-like proteinase-encoding region may be the cause for the aggregation of the product encoded by this region of ORF 1a. ...
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