The open reading frame (ORF) of HB–EGF cDNA encodes a 208 amino acid translation product that corresponds to the HB–EGF precursor [7](Fig. 2A). Mature soluble HB–EGF (box in Fig. 2A, solid box in Fig. 2B) is released from the membrane-anchored precursor by proteolytic cleavage [7...
(Michel & Westhof, 1990). Some group I introns contain anopen reading frame(ORF), which encodes information for mobility and splicing. Conventional group I ORFs are calledhoming endonucleasegenes (HEGs) and the ones present in plant/algal mitochondrial group I introns typically have distinctive ...
(1997). A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomo- nas reinhardtii encodes an essential protein. Mol. Gen. Genet. 253, 649-653.Boudreau et al., A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential ...
The longer RNA (RNA 1) has a single open reading frame (ORF) encoding an RdRp and the shorter RNA (RNA 2) has a single ORF encoding a coat protein (CP). Full size image Partitiviruses from the mycorrhizal fungi of Cypripedium belong to the genus Alphapartitivirus A BLAST search using...
The full-lengthBdorOBP2complementary DNA (cDNA) consisted of 621 nucleotides (nt), with an open reading frame (ORF) of 417 bp, and an encoded polypeptide of 139 amino acids. To determine the phylogenetic relationship betweenBdorOBP2and the 91 OBPs reported inBactrocera tau,Bactrocera cucurbit...
(UTR), at least 7 open reading frames (ORF1a, ORF1b, and ORF2–6), and a 3’ UTR [20]. The two large ORFs 1a and 1b occupy the 5’-proximal two-thirds of the genome coding for nonstructural proteins (nsps). ORF1a translation yields a replicase polyprotein (pp) la, whereas ...
CJIE: Campylobacter jejuni integrated elements ORF: Open Reading Frame PAM: Protospacer Adjacent Motif PHASTER: Phage Search Tool Enhanced Release RAST: Rapid Annotation using Subsystem Technology References Kaakoush NO, Castano-Rodriguez N, Mitchell HM, Man SM. Global epidemiology of campylobacte...
The Entry/Gateway® cloning procedures for generating epitope-tagged fusion proteins using the P-element destination vectors. The procedures of Entry/Gateway® cloning are illustrated in the diagram as two major steps. (1) In the first step, a fragment encoding the open reading frame (ORF) ...
Interestingly, only the protein encoded by the geneCZG_jg17822which was predicted to catalyzeO-methylation on 3’-position displayed the expected function (catalyzeO-methylation on 3-hydroxyl group) and was named CcOMT1. The open reading frame (ORF) ofCcOMT1was 1080 bp and encoded a putativ...
It was described earlier thathorAOMP locus in 26695 is composed of two open reading frames (ORFs) (HP0078/HP0079) whereas that in J99 is composed of one ORF (jhp0073) [27]. ThehorAlocus in all the hspEAsia strains shows apparent gene decay by fragmentation through various mutations (Fig...