b, GAD2-Cre mice injected with AAV-DIO-OxR2 displayed increased expression of OxR2 compared to mice injected with AAV-DIO-GFP as determined by ISH (two-tailed student’s t-test, n = 3 biologically independent mice, 2 slices per mouse, t(4)=4.417, p = 0.0069) (left). ...
AAV-Ef1α-DIO-ChR2-mCherry was bilaterally injected into the LH of orexin-Cre mice (orexinChR2 mice), and optical fibers were implanted in the bilateral SLD (Fig. 6a, b). Through patch-clamp recordings, we found that in orexinChR2 mice, optogenetic activation of the SLD orexin terminals...
Using a combination of circuit tracing, optogenetics, and patch-clamp electrophysiology in male and female orexincre mice, we showed a diverse response of LHox/dyn optical stimulation on VTADA neuronal firing, which is not mediated by fast transmitter release and is blocked by antagonists to KOR...
( REM) sleep depr i ved conditions, the central serotonin def i cient mice ( Petl— Cre/ Lmx1 b f l ox/ f l ox CKO mice) and small plat. for m method was used to establish mouse model of R E M sleep depr i vation. Immunohistochemi cal method was per f or m ed to ...
Here, we show that ectopic expression of HB〦GF, an important regulator of neurogenesis, in Nestin+ neuroepithelial progenitors with the Cre㎜oxP system leads to development of spontaneous middle age obesity in male mice accompanied by hyperglycemia and insulin resistance. The Nestin〩B〦GF mice ...
We recorded from TdTomato-labelled VLPOGABA/Gal neurons in Gal-IRES-Cre mice that had received injections of AAV-DIO-TdTomato into the VLPO. TdTomato-labeled VLPOGABA/Gal neurons expressed GABAergic markers, and as predicted, they were inhibited by NA. The majority of VLPOGABA/Gal neurons ...
Using hypocretin receptor-1 and hypocretin receptor-2 antagonists, hypocretin-1 and hypocretin-2 peptides, and hypocretin receptor-1 knockout mice, we investigated the role of the hypocretin system in cue- and context-dependent fear conditioning and extinction. Hypocretins were crucial for the ...
we used the already validated orexin-DTR mice25,46; the confirmatory histology shown in Fig.5awas performed as in ref.46. For SNc DA neuron recordings (Extended Data Fig.5a,b), we used DA transporter-Cre transgenic mice (The Jackson Laboratory 020080,Slc6a3tm1(cre)Xz/J). The animals we...
Orexin-Cre mice expressing hM3Dq in orexin neurons were intraperitoneally injected with saline or CNO (1.0 mg/kg). The arrowheads indicate triple co-localization of orexin, hM3Dq-mCherry and cFos. Scale bar is 50 μm. (C) Diagram illustrating the behavioral test protocol. AAV ...
(94.3 ± 2.1%, mean ± SEM for n = 3 mice; Fig.1b,g). Conversely, 96.8 ± 1.4% of CRE-positive cells also expressed TH, confirming high penetrance and specificity of the transgene, respectively. Next, to determine efficiency and specificity of CRE-mediated ...