The Michaelis constant, was estimated for both immobilized and free enzymes. Immobilized alginate-amylase had a higher Km (2.54 mM) than that of the free enzyme (1.12mM). It was evident that after immobilization, substrate affinity of α-amylase was decreased.Dr. Preeti Lahiri...
中文: 壳聚糖酶在经固定化后,最适温度为50℃,最适pH为4.5,并表现出比游离酶更高的热稳定性,固定化酶的米氏常数Km值为14.29 g/L; 更详细... 英文: Predicting optimal pH of alpha-amylase with percent of amino acid 中文: α-淀粉酶氨基酸含量与其最适温度的关系 更详细...分享...
The optimalpH optimal temperature of the laccase were 4. 5)进行梯度洗脱,可以获得较为纯化的漆酶,经研究,该菌株所产漆酶,以邻联甲苯胺为底物,最适pH值为4。 5) optimal pH 最适pH 1. For studying the relation between percentage of kinds amino acids andoptimal pH, we built alpha amylase's bank...
alpha‐amylaseOryza sativariceRVAsilver nitrateEndogenous α゛mylase interferes with accurate determination of the pasting properties of cereal flour. Silver nitrate (AgNO3) solutions at seven different concentrations (0.001 to 0.1 M) plus a deionised water (dH2O) control were compared for their ...
A new type of α-amylase starch substrate rendered insoluble by cross-linking and coloured by a dye marker, was shown to be hydrolyzed by α-amylase from Hyland standard serum, Versatol-E and Enzatrol. The various conditions for enzymatic hydrolysis of such a blue starch polymer were studied...
Optimizing the production of recombinant proteins is a problem of major industrial and pharmaceutical importance. Secretion of the protein by the host cell considerably simplifies downstream purification processes. However, for many proteins, this is als
Gelatinisation measurements showed that mechanical forces lowered the temperature required for complete gelatinisation. During hydrolysis experiments, high DEs were observed even if starch was not completely gelatinised during extrusion. Due to high substrate concentrations, the residual alpha-amylase ...
(1997). On-line fault diagnosis for optimal rice alpha-amylase production process of a temperature-sensitive mutant of Saccharomyces cerevisiae by an autoassociative neural network. Journal of Fermentation and Bioen- gineering, 83(5), 435}442....
The AmyA and AmyB had a similar melting temperature of 64掳C and the same catalytic efficiency (kcat/Km) of 2.0 脳 106 L/(mol min). AmyA and AmyB were slightly activated by 1 mM Co2+, Ca2+, or Na+, but inhibited by all other metal ions (K+, Mg2+, Fe3+, Fe2+, Zn2+, ...
Gelatinisation measurements showed that mechanical forces lowered the temperature required for complete gelatinisation. During hydrolysis experiments, high DEs were observed even if starch was not completely gelatinised during extrusion. Due to high substrate concentrations, the residual alpha-amylase ...