3.OPC-like亚群的位置特异性 通过对OPC-like无监督聚类得到3亚群,OPC-like-1表达经典OPC的marker基因,相比之下,OPC-like-2和OPC-like-3细胞表现出前OPC分化状态(图3a-f)。使用scRNA-seq数据集对OPC-like亚群特异性GRN的分析发现,SHOX2和OTX2等TF在OPC-like-1细胞中具有最特异性的活性(图3g)。OPC-like-2...
Cell proliferation was determined by [3H]thymidine incorporation, and Western blot analysis was performed for detection of alpha-SMA, a phenotypic marker of transdifferentiation into myofibroblast. When the cells were exposed to 3-isobutyl-1-methylxanthine (IBMX; 50-1000 microM), a nonselective PDE...
gmOPCs compared to wmOPCs. (e–i) OPC were differentiated for 3 (e,f, andh) and 6 days (g–i) and incubated with either (e) R-mAb, recognizing GalCer/sulfatide, or (f–i) double stained for MBP (red), a mature marker of oligodendrocytes (OLGs) and Olig2 (green), OLG lineag...
2A). As expected, we noted an upregulation of cancer stem cell markers LGR5 and CD44, as well as epithelial-to-mesenchymal transition marker ZEB1, in the spheroids (Fig. 2B). Hence, following confirmation for the enrichment of cancer stem-like cells in spheroids, we used these as a ...
图1OPC对象层次模型2OPC工具箱的基本工作原理(1)搜索可用的opc服务器为了建立与OPC服务器的连接,首先要知道OPC服务器的ID及其所在的主机名。1)在本地计算机上查询所有可用的OPC服务器。hostInfo=opcserverinfo(localhost)得到结果如下:hostInfo=Host:localhostServerID:1x4cellServerDescription:1x4cellOPCSpecification:DA2...
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TDP-43 KD in C/C astrocytes increased OPC proliferation and O4+ cell number (Figures S5A–S5C). Accordingly, increased MBP+ cell number and MBP+ area were detected in astrocyte-OPC co-cultures on nanofibers with TDP-43 KD C/C astrocytes compared with that in co-cultures with control C/...
其中,Host: 'localhost'表明搜索到的OPC服务器在本地计算机上;ServerID: {1x4 cell}表明在本地计算机上搜索到四个OPC服务器。四个OPC服务器所遵循的OPC规范都是DA2.0。ObjectConstructor是指四个OPC服务器的制造商。 2)验证返回的OPC详细信息: 将OPC服务器ID信息赋值给allSevers数组 allServers = hostInfo.Server...
(numbers) its4_294_967_295, that is 4GB minus 1 byte. An unimaginably huge size in 1989 when the first PKZIP spec was published. Not that big now. The internalsheet1.xmlfile will break the4GBlimit at about 1 million rows with 100 columns - with number cell only. Excel handles this...
兔抗NG2购自美国neuromarker公司;鼠抗A2B5购自美国Millipore公司;Edu试剂盒购自上海锐博公司;CY3标记羊抗兔IgG、FITC标记羊抗小鼠IgG购自美国Jackson公司。 2.混合胶质细胞培养 出生3d内SD大鼠乳鼠经75%乙醇消毒后,用眼科剪和眼科镊取脑,在PBS液中充分漂洗;把脑剔除脑膜后,脑组织用眼科剪剪成小块,置于37℃...