All laboratories used the multiplexed OPA (MOPA) as previously described (Burton 2006), and a detailed protocol can be found at http://www.vaccine.uab.edu. Briefly, ten microliters of diluted bacteria (four strains) were added to 20 µl of serially diluted serum. After incubation at room...
The protocol was registered at the US National Institute of Health (ClinicalTrails.gov Identifier: NCT03100162). Ethical approval was obtained from the Bioethical Committee of Poznan University of Medical Sciences (No. 871/2015) and written informed consent was obtained from all participants prior to...