2.4.2. Cellular viability by Neutral red uptake assay According to the NRU protocol (Anon. INVITTOX, 2008, DB-ALM protocol 78), after cell exposure to the target chemicals (24 h), cells were washed with prewarmed PBS (150 μl/well). The washing solution was removed and the neutral re...
All laboratories used the multiplexed OPA (MOPA) as previously described (Burton 2006), and a detailed protocol can be found at http://www.vaccine.uab.edu. Briefly, ten microliters of diluted bacteria (four strains) were added to 20 µl of serially diluted serum. After incubation at room...
After heating at 95 °C for 5 min, 3 μL of the denatured protein extract, 10 μL of each primary antibody (at 1:20 dilution), HRP-conjugated anti-mouse secondary antibodies, chemiluminescent substrate, and protein normalization solution were loaded into the assay plate wells. Anti-JAK2 and...