A 0.1M potassium phosphate buffer (PPB) solution containing 1gl 1 polyethylene glycol (PEG) was found to be optimal for minimizing the interference with BSA in the measurement. On-line monitoring for rt-PA is carried out on a simulated bioprocess in the range 0–20μgml 1 . The data ...
The data obtained showed good agreement with the conventional off-line enzyme linked immunosorbent assay (ELISA) data. The FIIA using o -phthalaldehyde (OPA) derivatization is a valuable tool for on-line monitoring of low concentrated protein products in biotechnical production processes....