https://mirror.oxfordnanoportal.com/software/analysis/ont-guppy_6.0.1_linux64.tar.gz CPU version https://mirror.oxfordnanoportal.com/software/analysis/ont-guppy-cpu-6.1.5-1.el7.x86_64.rpm Install Guppy using dpkg:: alien ont-guppy-cpu-6.1.5-1.el7.x86_64.rpm dpkg -i ont-guppy-cpu-...
DeepMod2在CPU上高效运行,可推断表观单倍型或甲基化调用。与闭源工具Guppy和Dorado相比,DeepMod2性能相当。总之,DeepMod2是快速、准确的开源DNA甲基化检测工具。 图展示了全基因组和RRMS纳米孔测序的reads和甲基化调用的IGV图,并比较了RRMS reads覆盖。使用DeepMod2,作者在HG002 RRMS中检测5mC,发现全基因组CpG基序...
The optional--sanitizeoption can be used to greatly reduce file size when files contain optional data from the Guppy basecaller that could in principle be regenerated by running Guppy. The files output when using thesanitizeoption will be identical in structure to those output by MinKNOW when liv...
There is also GPU version of basecaller, which is slightly worse and slower than guppy, butdoes notrequire compute capability 6.2 (anything which can run Pytorch 1.0 is good enough). If you want to use GPU version, we recommend setting up Conda environment with pytorch first. ...
$ guppy_basecaller-i single_fast5-s single_fast5-c dna_r9.4.1_450bps_fast.cfg--fast5_out on--num_callers4--cpu_threads_per_caller3### --fast5_out 默认是off,这里要选成on,这样就可以输出带event信息的fast5文件了 4、将basecalling得到的fastq文件合并,并建立索引 ...
from the Guppy basecaller that could in principle be regenerated by running Guppy. The files output when using the ``sanitize`` option will be identical in structure to those output by MinKNOW when live basecalling is disabled. NB `compress_fast5` will copy .fast5 files in order to compres...
There is also GPU version of basecaller, which is slightly worse and slower than guppy, but does not require compute capability 6.2 (anything which can run Pytorch 1.0 is good enough). If you want to use GPU version, we recommend setting up Conda environment with pytorch first. It can be...