A. Improved health. B. Reduced costs. C. Unforeseen health problems. D. Increased availability. 相关知识点: 试题来源: 解析 C。解析:文中提到“off-target effects might lead to unforeseen health problems”,即脱靶效应可能导致不可预见的健康问题。反馈 收藏 ...
Single-nucleotide mutation at the core sequence could abolish gene editing mediated by a however active sgRNA. With a great majority of the target sites, a kind of 'super' off-target gene editing was observed with both CRISPR/Cpf1 and CRISPR/Cas9. For a given target site, mutation at ...
The use of multiple editing techniques D. The process of choosing the target gene 相关知识点: 试题来源: 解析 A。在基因编辑领域,“off-target effects”指的是基因组中的意外变化。选项 B 是对目标基因的成功编辑;选项 C 是使用多种编辑技术;选项 D 是选择目标基因的过程,均不符合“off-target ...
reduced by four-fold the off-target effects of a CRISPR-Cas9 molecule that uses a guide ribonucleic acid (RNA) to find, snip and replace the mutated hemoglobin gene responsible for sickle cell disease. It does this without significantly reducing the desired on-target gene-editing. ...
Yang told Xinhua that off-target effects are the largest source of risks for gene-editing technology, but no method could accurately predict those risks previously. Many genetic diseases are caused by single-base mutations, according to the researchers. ...
CRISPR gene editing holds great promise to modify DNA sequences in somatic cells to treat disease. However, standard computational and biochemical methods to predict off-target potential focus on reference genomes. We developed an efficient tool called CRISPRme that considers single-nucleotide polymorphis...
What is a potential risk of gene editing? A. Off-target effects. B. Making organisms stronger. C. Increasing the intelligence of organisms. D. Making organisms more resistant to sunlight. 相关知识点: 试题来源: 解析 A。解析:“One concern is the possibility of off-target effects”指出基因...
你要明确的是你所说的non target是指的那个方面,基因层面?蛋白层面?还是生理层面的。我们曾在什么地方讨论过non target没有?没头没脑一句话丢出来,我没法回答你这个问题。@leafgreen8 【我理解的off target 是相对于On target 来说的。相对于目的基因target的表达,其他整合引起的影响可以称为off target或者非预期...
CRISPR Cas9是最新的基因编辑技术，可以定点的、定断的敲除/修饰基因，也可以在指定位置插入基因。“指定”是通过一段guide RNA来实现，但是这个guide RNA有一定的概率识别非“指定”的位置上，就叫脱靶（offtarget）。所以，你这个应该是genetic-offtarget 吧？虽然没见过这么个连接法，一般都是offtargrt ...
CRISPert: A Transformer-Based Model forCRISPR-Cas Off-Target Prediction CRISPR-Cas9 has emerged as a popular gene-editing technique due to its flexibility, precision, and ease of use. It is a complex that consists of a Cas9 pro... W Jobson Pargeter,R Backofen,VD Tran - Joint European Co...