由于O-糖蛋白 O-糖基化没有特定的目标氨基酸 motif 且目标氨基酸有 Ser 和 Thr 两个,O-糖基化位点的定位较为困难;需要高效的串级质谱解离方法在两个相邻 O-糖基化位点中间产生位点决定性碎片离子来对 O-糖基化位点进行准确定位。高能碰撞诱导解离(HCD,图...
3.4. O-linked glycosylation motif analysis All 492 and 17 O-linked positions of the proteome and endogenic peptidome, respectively, were included in the motif analysis, although the proteome and endogenic peptidome were searched separately. Analysis revealed three prevalent motifs in the proteome. In...
O-linked glycosylation recognition motifProvided herein are glycoproteins containing O-linked glycosylation recognition motifs and methods for use in the manufacture of, for example, conjugated vaccines.
These simple rules explain the glycosylation (or lack of it) on 21 of 22 Ser/Thr in the extracellular domain of GpA. 展开 关键词: glycophorin A O-glycosylation motif solid-phase Edman degradation DOI: 10.1093/glycob/3.5.429 被引量: 226 ...
In order to further investigate the dynamics betweenN- andO-linked glycosylation, we defined the three amino acids on each side of the glycosylated Asn within the consensus motif of NxS/T (x is not proline (P)) as the “position associated to N-sequon” (named N ± 1–3). There...
D. Prolonged incubation in PUGNAc results in increased protein O-linked glycosylation and insulin resistance in rat skeletal muscle. Diabetes 53, 921–930 (2004). Article CAS PubMed Google Scholar Wang, X. et al. O-GlcNAcase deficiency suppresses skeletal myogenesis and insulin sensitivity in ...
Our data cannot exclude that the mutant of the glycosylation site at the Asn56 of the α-subunit and the deleted mutant of the CTP-region including O-glycosylation sites is induced the structural alteration for rec-eCG proteins. Thus, we suggest that the O-glycosylation motif should ...
From the crystal structure, OGT is composed of three regions: a tripeptide repeat (TPR) motif at the amino (N) terminal domain, a catalytic domain at the carboxyl (C) terminus, and a nuclear localization sequence (NLS) connecting these two domains (Kreppel et al.1997; Lubas et al.1997)...
55. The obtained outer membrane glycosylation dataset allowed us to search for a sequence motif using bioinformatics prediction. Thus, we explored the nature of O-linked glycosylation inE.coliusing the MotifX algorithm56. We searched for overrepresented motifs in a nine amino acid sequence window ...
Cosmc is ubiquitously expressed and acts as a specific molecular chaperone assisting the folding and stability of core 1 synthase. Thus, it plays a crucial role in the biosynthesis of O-linked glycosylation of proteins. Here, we show that ablation of Cosmc in the exocrine pancreas of mice cause...