Sequences without this motif were filtered out as non-N-glycopeptides. Furthermore, three additional criteria were applied to remove sequences that may fail to be detected in SRM studies. First, the molecular mass of the peptide must be between 400 and 4000 Da. Second, the length of the ...
The newly generated spectrum file was searched against a motif (N-X-S/T/C, where X is not P)-containing peptide database with the search engine MS-GF+ 27, which is an open-source software and is already integrated in Glyco-Decipher. Spectrum expansion was applied to increase the spect...
The motifs and sequence structures analysis showed that the significantly regulated N-glycosylation sites were concentrated within [NxT] motif, and 79.5% of them were located on the random coil that is always on the protein surface and flexible regions, which could facilitate protein glycosylated ...
In contrast, targeted analysis of IgG glycosylation would miss the observation of a sialyl-Lewis motif, for the simple reason that none of the IgG glycoforms contain this epitope20. The profiling of a complex sample, such as TSNG, can be performed using a combination of separation and ...
P (where !P is not proline) and rarely the N-X-C motif or nonconsensus sequences. Combining the FASP approach with analysis of subcellular glycosite localization reveals that the sites always orient toward the extracellular space or toward the lumen of ER, Golgi, lysosome, or peroxisome. ...
The co-occurrence of a molecular assembly motifa double-pillared parallel and antiparallel hydrogen bonding patternis hitherto unknown in the crystal structures of N-glycoprotein linkage region models and analogs.doi:10.1080/07328303.2011.631237Srivastava, Amrita...
Motif-X analysis suggested that [NxT], [NxS] and [NS] were significantly conserved and enriched. During leaf senescence, proteolysis-related proteins were predominately N-glycosylated by gene ontology analysis. Meanwhile, most identified glycoproteins were enriched in photosynthesis and N -glycan ...
Motif-X analysis suggested that [NxT], [NxS] and [NS] were significantly conserved and enriched. During leaf senescence, proteolysis-related proteins were predominately N-glycosylated by gene ontology analysis. Meanwhile, most identified glycoproteins were enriched in photosynthesis and N-glycan ...
moieties were released enzymatically, fluorescently labeled and analyzed by CE-LIF.In the positive acute-phase proteins, haptoglobin, α1-antitrypsin, and α1-antichymotrypsin, an increase of antennarity and LewisX motif could be measured in EOC patients on tri- and/or tetraantennary N -glycans....
N-glycosylation at Asn(491) in the Asn-Xaa-Cys motif of human transferrin. FEBS Lett. 576, 51–56 (2004). 46. Satomi, Y., Shimonishi, Y., Hase, T. & Takao, T. Site-specific carbohydrate profiling of human transferrin by nano-flow liquid chromatography/electrospray ionization mass ...