通过在体外修饰这些肽以产生O-GlcNAcylated或未修饰的肽。使用表面等离子体共振(SPR)分析,确认了O-GlcNAcylated肽和importin α3之间的直接相互作用,但未修饰的肽与importin α3之间没有直接的相互作用(图4G)。这些结果表明O-GlcNAc修饰可以显著增强SRPK2与importin α蛋白的相互作用,表明importin α可能是O-GlcNAc修...
O型GlcNAcylated 。 匿名 2013-05-23 12:23:18 O-GlcNAcylated。 匿名 2013-05-23 12:24:58 O-GlcNAcylated。 匿名 2013-05-23 12:26:38 O-GlcNAcylated。 匿名 2013-05-23 12:28:18 O-GlcNAcylated.热门同步练习册答案初中同步测控优化设计答案 长江作业本同步练习册答案 名校课堂助教...
O -GlcNAcylated peptides and proteins for structural and functional studies 来自 Semantic Scholar 喜欢 0 阅读量: 104 作者:AT Balana,SP Moon,MR Pratt 摘要: O-GlcNAcylation is an enzymatic post-translational modification occurring in hundreds of protein substrates. This modification occurs through ...
We have found that this linker performs the best in the CuAAC reaction, enabling the robust enrichment of potentially O-GlcNAcylated proteins followed by a simple cleavage reaction to retrieve these proteins from streptavidin beads. Access through your organization Check access to the full text by...
O-GlcNAcylated in vivo, we examined the O-GlcNAcylated ChREBP within the mesangium of STZ-induced diabetic rats exhibiting significant hyperglycemia using an in situ proximity ligation assay with anti-ChREBP and RL-2 antibodies, to make ChREBP exhibit fluorescence when it is O-GlcNAcylated...
Click-iG_ Simultaneous Enrichment and Profiling of Intact N-linked, O-GalNAc, and O-GlcNAcylated Glycopeptides Click-iG 一种多类型完整糖肽的鉴定方法 Click-iG:一种多类型完整糖肽的鉴定方法 Click-iG:一种多类型完整糖肽的鉴定方法 [Click Click on Compound Light Microscope. Then click … click iG ...
The level of UDP-GlcNAc, the donor of the sugar moiety forO-GlcNAcylation processes, was measured using high-pH anion exchange chromatography.O-GlcNAcylated proteins were enriched and purified utilizing either click chemistry labelling or adsorption on succinyl-wheat germ agglutinin beads. Proteins ...
We found O-GlcNAcylated site in ncOGT outside of TPR by using Q-TOF MS. Now we focus on the activities and function of ncOGT by mutagenesis studies. This ongoing effort would give us clear understanding of the key enzyme of O-GlcNAc metabolism and how the O-GlcNAc modification may be...
O-GlcNAcylated c-Jun antagonizes ferroptosis via inhibiting GSH synthesis in liver cancer Ferroptosis is a metabolism-related cell death. Stimulating ferroptosis in liver cancer cells is a strategy to treat liver cancer. However, how to eradicat... Y Chen,G Zhu,Y Liu,... - 《Cellular Signal...
The known O-GlcNAcylated proteins exert a broad range of functions including transcriptional regulation, macromolecular complex assembly, intracellular transport,... J Wang,M Torii,H Liu,... - 《Bmc Bioinformatics》 被引量: 129发表: 2011年 加载更多来源...