The natural processing and presentation of this epitope was demonstrated by recognition of an NY-ESO-1+ melanoma cell line by T cells with specificity for p134-148. The pentadecamer p134-148 was able to induce CD4+ responses in 4/38 cancer patients tested. However, no strict correlation ...
“g/m1).NY—ESO一1gene were RT—PCRandindirectimmunofluorescencemethods.ResultsTherecombinant detected proteinexpression by WaSconstructedandtheHLA—A2 celllinewith NY-ESO·1 positive highly pCDNA3.1/NY—ESO一1plasmid HepG2 and wasscreened.ConclusionANY—ESO一1 celllineisestab— gene proteinexpression...
To test the antitumor effect of DC-SNU sensitized PBMCs, we constructed a stable NY-ESO-1 expressing cell line MC38NY-ESO-1 based on mouse colon carcinoma MC38 cells using lentivirus transfection method. The puromycin-resistant cells were sorted with flow cytometry due to GFP expressed by MC...
Cell line authentication The soft-tissue sarcoma cell lines SW982 (HLA-A2-positive NY-ESO-1-positive), SYO-1 (HLA-A2-negative NY-ESO-1-negative), Fuji (HLA-A2-positive NY-ESO-1-negative) and MLS-1765-92 (HLA-A2-negative NY-ESO-1-positive) were provided by Prof. H. Shiku (Mie U...
Gene expression cut-off values were calculated on the basis of positive Gerl and LNCap cell RNA. The MAGE-A3 and PRAME expression level of the 1% Gerl (equivalent to 0.5 ng of RNA), normalised by the beta-actin expression level of the 100% Gerl (equivalent to 50 ng of RNA), wa...
DAC treatment markedly upregulated the expression of PD-L1 in the 1872 Col cell line at the total protein level (Fig. 5A) and at the surface expression level (Fig. 5B). However, PD-L1 had no marked or significant induction/upregulation in the 1076 Col cells (Data not shown). Fig. 5...
We generated a novel murine tumor cell line (which we named, “CIN”) by genetically modifying the parental CT26 colon cancer cells to express human NY-ESO-1. CIN cells were plated at near single-cell density to screen clones for uniform expression level of NY-ESO-1. CIN clones expressing...
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. Click here to download PDF datasheet. Target Protein Principal Names: CTAG1B, cancer/testis antigen 1B, CTAG, CTAG1, ESO1, LAGE-2, LAGE2B, NY-ESO-1, New York esophageal squamous cell ...
展开 关键词: Humans Lymphocytes, Tumor-Infiltrating Cell Line Tumor Cells, Cultured Melanoma Monophenol Monooxygenase Isoenzymes Intramolecular Oxidoreductases Proteins Membrane Glycoproteins DOI: 10.4049/jimmunol.168.2.951 被引量: 215 年份: 2002 收藏 引用 批量引用 报错 分享 全部...
Advanced-stage non-small cell lung cancer: advances in thoracic oncology 2018 J. Thorac. Oncol., 14 (2019), pp. 1134-1155 View PDFView articleView in ScopusGoogle Scholar [4] P. Martinez, S. Peters, T. Stammers, J. Soria Immunotherapy for the first-line treatment of patients with meta...