Up-regulation of nuclear protein import by nuclear localization signal sequences in living cells. FEBS Lett. 442:235-240.Tachibana, T., Hieda, M., and Yoneda, Y. (1999) Up-regulation of nuclear protein import by nuclear localization signal sequences in living cells. FEBS Lett. 442, 235-...
核定位信号 Nuclear localization sequence(NLS)Nuclear localization sequence A nuclear localization signal or sequence(NLS)is an amino acid sequence that'tags’a protein for import into the cell nucleus by nuclear transport.Typically,this sig-nal consists of one or more short sequences of positively ...
Retroviral vector particles are provided which contain: 1) oncoretroviral gag, pol, and env proteins, including an oncoretroviral gag capsid protein which has been mutated so as to contain a nuclear localization signal (NLS) sequence; an... JM Mason,SP Kennedy,SA Fidel 被引量: 49发表: 1996...
All Four Homochiral Enantiomers of a Nuclear Localization Sequence Derived from c-Myc Serve as Functional Import Signals The information that targets a protein to the nucleus often consists of a short cluster of basic amino acids called a nuclear localization sequence (NLS). ... ACS Saphire,SJ ...
A mutation in the nuclear localization signal of squamosa promoter binding like-protein 9 (SPL9) delays vegetative phase change by disrupting its nuclear localization. The juvenile-to-adult phase transition is a critical developmental process in plant development, and it is regulated by a decrease ...
some of them have nuclear localization function, while others do not. For example, a PY-NLS-like sequence was predicted to be encoded by residues 259–306 of poly(A) binding protein nuclear 1 (PABPN1). This putative NLS is dominated by basic amino acids, but has no nuclear positioning fu...
(i) the classical pathway, which involves the classical nuclear localization sequences (cNLSs) and the receptors importin-α/karyopherin-α and importin-β/karyopherin-β1; and (ii) the karyopherin-β2 pathway, which employs the proline-tyrosine (PY)-NLSs and the receptor transportin-1/...
The highest level of antibodies raised against PCV2 ORF2 Cap protein was detected with pcDNA3-ORF2 plus VTN. Contrary to the in vitro results, VTN delivered pDNA effectively in vivo without Lipofectamine. In summary, the nuclear localization signal sequence of porcine circovirus type 2 ORF2 ...
Cells were transfected using Lipofectamine 2000 reagent (Life Technologies, Cat. 11668030). Nuclear actin was visualized by transfecting cells with Actin-Chromobody®plasmid (Actin-nanobody, fused to TagGFP containing a nuclear localization sequence, (ChromoTek). Emerin-pEGFP-C1 plasmid was obtained...
Transportin 1 (TNPO1, also known as karyopherin β2) and Transportin 2 (TNPO2, also known as Importin 3) are highly homologous (85% sequence identity) and well-characterized importins that bind the PY (proline-tyrosine) nuclear localization signal (NLS) of cargo proteins.99A typical PY ...