viruses Review NSP16 2 -O-MTase in Coronavirus Pathogenesis: Possible Prevention and Treatments Strategies Li-Jen Chang 1,2 and Tsung-Hsien Chen 2,* 1 Division of Gastroenterology and Hepatology, Department of Internal Medicine, Ditmanson Medical Foundation Chia-Yi Christian Hospi...
Ethics statement The PAMs used in this study were from 6-week-old PRRSV-negative piglets. Animals were euthanized, and carcasses were treated innocuously. The work was approved by the Institutional Animal Care and Use Committee of Sun Yat-sen University. Cells and viruses African green monkey ki...
HP-PRRSV strain JXwn06 (a highly pathogenic PRRSV strain isolated in Jiangxi Province; GenBank accession, EF641008.1) was propagated in PAMs, and typical PRRSV strain CH-1a (the first type 2 PRRSV strain isolated in China; GenBank accession, AY032626.1) was propagated in Marc-145 cells. Vir...
The three-dimensional crystal structure of NSP15 was retrieved from the Protein Data Bank (PDB ID: 6VWW) [19]. To perform the docking studies, the protein structure was optimized using the protein preparation wizard available in the Schrödinger software suite [20,21]. The protein model prepa...
The electron density maps are available from the Electron Microscopy Data Bank (EMDB) under accession codes EMD-11024 and EMD-11023 for single and double rings, respectively. All other data generated or analysed in this study are available from the corresponding author upon reasonable request. ...
Porcine epidemic diarrhea virus (PEDV), a member of the α-coronavirus genus, can cause vomiting, diarrhea, and dehydration in piglets. Neonatal piglets infected with PEDV have a mortality rate as high as 100%. PEDV has caused substantial economic losses to the pork industry. Endoplasmic reticulu...
The protein structure of the Nsp1 N-terminal domain was taken from the Protein Data Bank using the accession code 7K7P. The Δ82–85 protein structure was built with the Residue and Loop mutation tool available in Maestro using the Nsp1 N-terminal domain (7K7P) as a template. The 5′...
able to show with bad Marlins teams in an uncommonly large stadium. Looking strictly at the distance and location of his batted balls last season, between 15 and 20 of Realmuto's deep flyouts and lineouts would have been extra-base hits — half of them home run...