NSC proliferation was assessed by MTT assay and protein expressi on (Western) of the NSC marker (nestin). Neurogenesis was assessed by expression of neuronal (Tuj1) or astrocyte (GFAP) cell markers. Mechanisms of TNFα action were explored by assay of bHLH proliferative (Hes1) and pro...
(Catalog #NL007; red). Astrocyte lineage cells (B) were detected with a Sheep Anti-Human GFAP Antigen Affinity-purified Polyclonal Antibody (Catalog #AF2594) followed by the NL557-conjugated Donkey Anti-Sheep IgG Secondary Antibody (Catalog #NL010; red). Oligodendrocyte lineage cells (C) were...
(a marker of differentiation of NSC-34 cells towards the MN-like phenotype) expression evaluated by Western Blotting analysis. HSP90 protein was used as a control. (C): Relative mRNA levels of the cholinergic markers AChT (acetylcholine transporter), AChE (acetylcholinesterase) and VAChT (...