Keap1:Nrf2 Inhibitor Screening Assay Kit使用荧光偏振(FP)检测技术,在该技术中使用荧光素标记的Nrf2短肽与Keap1结合形成复合物,复合物的形成会造成FP值上升。在抑制剂存在的条件下,则不会产生高FP。可以通过读取FP值检测抑制剂的效应。 03应用 酶动力学研究、小分子抑制剂筛选、高通量筛选。 04验证数据 左图:Ke...
lifespan of worms, decreasing the accumulation of lipofuscin, inhibiting ROS production, and increasing the proportion of skn-1 in the nucleus. With the Nrf2 inhibitor ML385, we verified that quercetin and hyperoside prima...
KEAP1-Nrf2 Inhibitor Screening Assay Kit 96reactions 非常适合研究酶动力学和筛选药物发现和高通量应用的小分子抑制剂 结果赏析: 左图:Keap1与Nrf2的结合。右图:Nrf2抑制剂抑制Keap1-Nrf2结合。 结合情况使用Keap1:Nrf2抑制剂筛选试剂盒(BPS Bioscience #72020)进行检测。Nrf2抑制剂KI696与Nrf2肽和Keap1在逐渐增加...
摘要:目的 探讨小檗碱对于Erastin诱导小鼠海马神经元HT22细胞的铁死亡的保护作用及其可能机制。方法 以HT22小鼠海马神经元细胞为研究对象,分为对照组、Erastin模型组、Erastin+30 μmol/L BBR组、Erastin+60 μmol/L BBR组。采用CCK-8法、特异性 Fe2+ 荧光探针、荧光染料(DAPI)检测和荧光探针(H2DCFH-DA)检测各实验...
南京医科大学第一附属医院肝胆中心吕凌教授团队于2022年6月于《Hepatology》发表了题为“Macrophage nuclear factor erythroid 2-related factor 2 deficiency promotes innate immune activation by tissue inhibitor of metalloproteinase 3-medi...
whereas EP1 activation exerted opposite effect. Genetic depletion of EP1 in cardiomyocytes worsens DOX-induced cardiac injury in mice, which was efficiently rescued by the ferroptosis inhibitor Ferrostatin-1 (Fer-1). Mechanistically, EP1 activation protected cardiomyocytes from DOX-induced ferroptosis by ...
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Inhibitor of Nrf2 (INrf2 or Keap1) protein degrades Bcl-xL via phosphoglycerate mutase 5 and controls cellular apoptosis. J Biol Chem. 2011;286:44542–56.Niture S.K., Jaiswal A.K. Inhibitor of Nrf2 (INrf2 or Keap1) protein degrades Bcl-xL via phosphoglycerate mutase 5 and controls ...
lifespan of worms, decreasing the accumulation of lipofuscin, inhibiting ROS production, and increasing the proportion of skn-1 in the nucleus. With the Nrf2 inhibitor ML385, we verified that quercetin and hyperoside primarily protected the cells against oxidative damage via the Nrf2 signalling ...
we demonstrate that Nrf2 regulated STING levels by controllingTMEM173stability. The ability of the Nrf2 inhibitor ML385, which works by blocking Nrf2-DNA binding35, to abrogate Nrf2-mediated suppression of STING suggests that the effect of Nrf2 on STING is not direct but is likely to work throu...