以胶质瘤组织lncRNA NR2F2-AS1相对表达量的平均数(2.89)为临界值,将患者分为lncRNA NR2F2-AS1低表达组(<2.89,47例)与lncRNA NR2F2-AS1高表达组(≥2.89,56例)。LncRNA NR2F2-AS1低表达组5年累积生存率为51.06%(24/47),lncRN...
增殖凋亡目的:研究lncRNA NR2F2-AS1对胃癌细胞增殖,凋亡的影响,并探讨其机制.方法:运用qRT-PCR法检测细胞中NR2F2-AS1,miR-425-5p的mRNA表达情况;将pcDNA组(转染pcDNA),pcDNA-NR2F2-AS1组(转染pcDNA-NR2F2-AS1),sh-NR2F2-AS1组(转染sh-NR2F2-AS1),sh-NC组(转染sh-NC),anti-miR-NC组(转染anti-miR...
Cell proliferation data showed that NR2F2-AS1 overexpression led to increased, while NR2F2-AS1 siRNA silencing led to proliferation rate of PC cells. Moreover, NR2F2-AS1 also showed positive effects on cell cycle progression. In addition, CDK4 overexpression reduced the effects of NR2F2-AS1 ...
采用荧光定量聚合酶链式反应(qRT-PCR)对子宫内膜癌组织与细胞中miR-129-5p和LncRNA NR2F2-AS1的表达水平进行检测;用流式细胞术与MTT比色法(MTT法)对子宫内膜癌细胞(Ishikawa)凋亡与增殖水平进行检测;用蛋白质印迹法(Western blot)对细胞中B淋巴细胞瘤-2基因(Bcl-2),兔抗人单克隆抗体(Bax),p21基因(p21),...
使细胞中CyclinD1和Bcl-2含量降低(P<0.05),p21和Bax含量升高(P<0.05);lncRNA NR2F2-AS1靶向负调控miR-129-5p的表达;抑制miR-129-5p表达逆转了干扰NR2F2-AS1表达对U251细胞增殖,凋亡的作用.结论干扰lnc RNANR2F2-AS1通过靶向促进miR-129-5p表达抑制胶质瘤U251细胞增殖,诱导细胞凋亡.LncRNA NR2F2-AS1可能...
In addition, NR2F2-AS1 silencing increased OSCC cell proliferation rate while overexpression of miR-494 and NR2F2-AS1 decreased OSCC cell proliferation. Furthermore, miR-494 overexpression attenuated the effects of NR2F2-AS1 silencing on cell proliferation. CONCLUSION :NR2F2-AS1 may inhibit miR-...
Rac1stemnessBackground: Zhang et al. characterized a novel oncogenic long noncoding RNA (lncRNA) named NR2F2-AS1 in lung cancer. In this study, the role of lncRNA NR2F2-AS1 in clear cell renal cell carcinoma (ccRCC) was explored.Chen, LinZhang, DongxingDing, TaoLiu, FengXu, Xiaolin...