事实上,在MCF10AT中,敲除PTBP1以及过表达NAS1完全阻断了NR2F1通过NAS1表达;而在CA1h-P2中,PTBP1与NAS1敲除后,NR2F1蛋白水平恢复(Fig. 5c),证实了PTBP1介导了NAS1在NR2F1调控中的作用。 进一步分析PTBP1如何调控NR2F1...
lncRNA NR2F1-AS1microRNA-493-5pITGB1目的非小细胞肺癌(Non-small-cell lung cancer,NSCLC)约占肺癌(Lung cancer)的85%,是最为常见的恶性肿瘤,已成为世界上发病率及死亡率最高的恶性肿瘤之一.高表达的ITGB1参与NSCLC增殖,迁移和侵袭等恶性进程.miR-493-5p作为抑癌miRNA,在NSCLC中与肿瘤的不良预后呈负相关....
事实上,在MCF10AT中,敲除PTBP1以及过表达NAS1完全阻断了NR2F1通过NAS1表达;而在CA1h-P2中,PTBP1与NAS1敲除后,NR2F1蛋白水平恢复(Fig. 5c),证实了PTBP1介导了NAS1在NR2F1调控中的作用。 进一步分析PTBP1如何调控NR2F1的表达,在排除了PTBP1调控NR2F1启动子活性的可能性之后,通过RIP和融合蛋白免疫沉淀试...
NR2F1-AS1PTCSOX12Papillary thyroid carcinoma (PTC) is an aggressive histological subtype of thyroid carcinoma (THCA), whose occurrence rate is high. The participation of long noncoding RNAs in the pathologies of cancers has attracted significant attention during the past decades. The purpose of ...
Background. LncRNA NR2F1-AS1 has been identified as an oncogene in some human tumors, such as breast cancer, nonsmall cell lung cancer, and esophageal squamous cell carcinoma. Nonetheless, whether NR...
LncRNA NR2F1-AS1 has been identified as an oncogene in some human tumors, such as breast cancer, nonsmall cell lung cancer, and esophageal squamous cell carcinoma. Nonetheless, whether NR2F1-AS1 is involved in the progression of gastric cancer (GC) remains unknown. Methods. The expression ...
CCK8与LncRNANR2F1AS1和甲状腺癌细胞的增殖和迁移研究 甲状腺癌(TC)是全球最常见的内分泌恶性肿瘤,约占头颈部恶性肿瘤的三分之一。近几十年来,全球甲状腺癌发病率和死亡率不断上升,有大量的危险因素与TC的发生和发展密切相关,如遗传因素、环境暴露和表观遗传改û...
Fig. 2 NR2F1-AS1 (NAS1)促进乳腺癌的转移性休眠 3.NAS1促进EMT和侵袭,但抑制癌细胞的肿瘤启动能力 为了分析为什么NAS1会促进癌细胞生长,但抑制肺转移,作者首先分析了癌细胞的EMT状态和侵袭性。从细胞形态和各种EMT标记物的表达可以看出(Fig. 3a),在CA1h-P2 CSC亚群中,NAS1敲低可部分诱导上皮间充质转化(...
(P<0.05);GSK-3β是miR-212-3p的靶基因,且过表达miR-212-3p的RA-SF中GSK-3β蛋白表达降低(P<0.05);敲减miR-212-3p降低了敲减NR2F1-AS1对RA-SF活性,凋亡及PCNA,cleaved-Caspase-3蛋白表达的影响.该研究提示,NR2F1-AS1可能通过靶向调控miR-212-3p/GSK-3β轴促进RA-SF增殖并阻碍其凋亡,其可能成为RA...
Moreover, NR2F1-AS1 forms a complex with SRSF1 to upregulate NR2F1 expression, facilitating its binding with ESR1 to sustain hormonal receptor expression and enhance proliferation in ENZ-R cells. Furthermore, HnRNPA2B1 interacts with NR2F1 and NR2F1-AS1, assisting their packaging into exosomes...