研究表明,MRD评估可以明确疾病的治疗反应,有助于完善预后风险分层,MRD持续阳性具有不良预后和较高的复发风险;此外,对CR患者进行长期MRD监测可能有助于及早发现白血病复发[1]。AML1-ETO、PML-RARA、CBFB-MYH11等融合基因作为AML的特异性MRD标志物已得到广泛应用,缺憾在于大...
建立检测多种NPM1突变的AＲMS-PCＲ的方法。通过对该方法的检测范围、灵敏度的评估及与直接测序法的比较，判断该方法的可行性。结果表明，成功构建NPM1基因野生型和突变型A 、B 、C 、D 5种重组质粒，经测序鉴定和目的片段一致。AＲMS-PCＲ方法可以检测到ABCD 4种突变体，检测范围在103copies /ml －109copies...
Assessment of measurable residual disease (MRD) by real time quantitative polymerase chain reaction (RT-qPCR) forNPM1mutant transcripts [11] can be combined with the ELN risk stratification to inform therapeutic decisions, e.g. helping to select patients who may benefit from allogeneic haematopoietic...
MRDMinimalresidualdisease微小残留病灶 4 急性髓系白血病FLT3-ITD及NPM1基因突变分析及其预后意义 摘要 背景:急性髓系白血病(AML)是一组具有高度异质性的恶性克隆性疾病,细 胞及分子遗传学异常是其致病基础也是预后相关重要因素。近年来先后发现多个 基因突变,这些分子遗传学异常的特点及预后影响已有报道。最常见的是 FL...
1723 Background: Quantification of minimal residual disease (MRD) by multiparameter flowcytometry (MFC) in patients with acute myeloid leukemia (AML) provides significant prognostic information. Previous analyses have shown that positivi... D Sauter,K Ringel,J Braess,... 被引量: 1发表: 2010年 ...
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Ancillary testing during the initial workup of acute myeloid leukemia (AML) is largely performed using aspirated materials. We utilized multiplex immunofluorescence (MIF) imaging with digital image analysis to perform an in situ analysis of the microenvi
Only FLT3-ITD co-mutation did not have a deleterious effect on acute myeloid leukemia patients with NPM1 mutation, but concomitant with DNMT3A co-mutation or a < 3log reduction of MRD2 predicted poor survival Article17 September 2024 ...
DNA-based NGS assays for mutated NPM1 MRD testing may have several advantages but require validation. We performed NPM1 MRD detection in blood collected from patients with AML during first CR (CR1) prior to alloHCT on a subset of patients from the Pre-MEASURE study (PMID: 36881031) using ...
Through our study encompassing a large patient cohort of 140 patients and nearly 350 testing data points, we confirm the feasibility and clinical utility of NPM1 MRD evaluation by NGS. The significantly poorer survival in patients who were MRD+ at any time during their disease provides a ...