Recordings were performed at room temperature and extracellular solution was exchanged at a flow rate of 3–4 mL/min. AMPA receptor-mediated EPSCs were recorded at -70 mV, and NMDA receptor-mediated EPSCs were recorded at + 40 mV. Peak current for NMDA receptor-mediated EPSCs was taken ...
When at sea level and breathing room air, the partial pressure of O_2 in inspired air is a. 760 mm Hg b. 40 mm Hg c. 100 mm Hg d. 160 mm Hg e. 150 mm Hg Aortic blood pressure is highest during: a. Atrial systole...
We added additional 1.8 mL of tissue lysis solution to rinse the dish, transferred all the tissue fragments from the Petri dish into a clean 5 mL low-binding tube (Eppendorf, cat. no. 0030108.310), and rotated the tube at 20 rpm at room temperature for 15 min. We stopped the...
Samples were boiled for 5 min in a water bath and cooled to room temperature. DNase (0.5 mg per ml) was added and CE counted using a hemacytometer. CE formation was calculated as a percentage of input cells. Analysis of nucleosomal DNA fragmentation DNA was isolated and labeled as ...
fluorescent secondary anti-rabbit antibody (1:500) for 1 hour in a humidified chamber at room temperature. The sections were counterstained with 0.001% (v/v) 4,6-diamidino-2-phenylindole dihydrochloride (DAPI) and mounted in Vectashield solution. Tissue preparations from adult ovaries were ...
Four parts conditioned medium was incubated at room temperature with 1 part of McCoy's medium or 1 part of a 1:10 dilution of antiserum for 1.5 hr before assay against 105 nonadherent low density human bone marrow cells or 7.5 × 104 mouse bone marrow cells. Human-cell-derived conditioned...
After washing in 0.01M PBS, the cells were incubated in 0.5 % H2O2 solution to inactive endogenous peroxidase, nonspecific binding was blocked with normal goat serum at room temperature for ten minutes.The cells were incubated with anti-P27kip1 protein monoclonal antibody (impromptu type, Zhong ...
Immunoglogulin ITf: Interstitial tissue-enriched fraction IR: Insulin receptor SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis STf: Seminiferous tubule-enriched fractions PBS: Phosphate buffered saline PMSF: Phenylmethylsulfonyl fluoride RT: Room temperature wt: Wild type ...
repel WWOXd cells. Further, we determined here that in response to stress stimuli at room temperature, WWOXf cells undergo non-apoptotic bubbling cell death (BCD) initiating from the nucleus at room temperature39,40. WWOXf cells have a functional Ca2+influx system. In contrast, WWOXd cells ...
Cells were washed in PBS, and 750 µl of hexane:isopropanol (2:3) were added to each well and incubated rocking for 10 min at room temperature. The organic solvent phase was collected and dried. Lipids were resuspended in 40 µl of chloroform, spotted on a TLC plate, and developed ...