Here, to determine for the first time the lethal electric field levels in vivo in healthy and in chronically infarcted pig hearts, we performed in vivo experiments using a focal PFA catheter. We assessed the time course of lesion development in healthy animals using intracardiac electrograms (iEGM...
The cholangioids were placed in 25 μl of Dispase (Corning) and incubated at 37 °C, 5%CO2 for 2 h to dissociate the cells. Single cells were counted using a hemocytometer and Trypan blue. The cholangioid size was calculated by averaging the vertical, horizontal, and diagonal diameters ...
particularly in muscle with decreased GLUT4 protein content22,26,27,35,50,51,52,53. Insulin resistance in skeletal muscle of rats lacking TBC1D4 is rescued by re-introducing normal GLUT4 protein levels35. We propose that the insulin resistance characterizing skeletal muscle lacking TBC1D4 is pr...
The results indicate that population inversions between the (040) and (001) energy levels of CO2and,to a lesser degree,between the (200) and (001) levels,can be created in the vibrational nonequilibrium flow behind a normal shock front. The properties of these inversions as a possible laser...
Major determinants of whole-body energy balance were evaluated by the Yale School of Medicine Mouse Metabolic Phenotyping Center using the Comprehensive Lab Animal Monitoring System with Oxymax (Columbus Labs), including VO2, VCO2, activity, feeding, and drinking behavior. Body composition was determin...
After all this, CO2 emissions last year climbed 7% to a record high, despite trillions being spent. The reality is that government’s environmental policies can’t suck and blow at the same time. Shortages and high energy prices will persist making inflation worse this time as climate ...
The lactate production is associated chiefly with the interstitial and Sertoli cells in which glucose is oxidized to CO2 in small amounts but predominantly through the pentose cycle and pyruvate carboxylation pathways necessary for maintaining the citric acid cycle [22]. The report of a negative ...
Peripheral blood mononuclear cells were washed twice with Dulbecco's PBS, resuspended in RPMI with 2% human AB serum, and incubated for 2 hours at 37°C and 5% CO2. Nonadherent cells were removed by gentle washing with RPMI, and the remaining adherent monocytes were cultured in RPMI with 2%...
We grew all the cell lines at 37 °C in 5% CO2 air, except for S2 cells, which were grown at 28 °C in a non-humidified, ambient air-regulated incubator. Prostate samples We prospectively collected six prostatectomy samples (P1-P6) from male patients aged 43–65 and operated for ...
Cell Culture AsPc1 and BxPc3, two human pancreatic carcinoma cell lines, and HT29, a human colon carcinoma cell line, were purchased from American Type Culture Collection (Rockville, Maryland), kept in a humidified incubator at 37° C in 5% CO2 atmosphere and cul- tured in Iscove's ...