Similar results were obtained from testing the binding to human epithelial type 2 (HEp-2) cell lysates, a commonly used assay to determine antibody polyreactivity (Figure 3F). We conclude that the monoclonal antibodies that we isolated are not polyreactive and show strong binding to MMPs, ...
Lines of evidence suggest that Bcl-XLhas a strong binding affinity with p53, and can suppress p53-mediated tumor cell apoptosis.26In this study, FLAG-tagged Bcl-XLand HA-tagged p53 were co-transfected into cells; immunoprecipitation experiment was performed by using FLAG antibody to immunoprecipita...
(E) Representative HEp-2 cell staining with a commercial anti-nuclear antibody as positive control revealed nuclear binding (top). S1-reactive non-neutralizing mAb CV38-148 exhibited cytoplasmatic binding (middle). Neutralizing mAb CV07-209 showed no binding (bottom). All mAbs selected for detail...