sense strand是指编码链 不作为转录 的另一条DNA链为编码链,又称有意义链. antisense strand是指反义链 在基因的DNA双链中,转录时作为mRNA合成模板的那条 DNA单链叫做模板链或反义链. 还有的两个就不知道了. 分析总结。 antisensestrand是指反义链在基因的dna双链中转录时作为mrna合成模板的那条dna单链叫做...
6e). We propose a model wherein dCas9 binding could be reduced on the template strand due to competition with Pol II-mediated transcription, rendering KRAB ineffective. By contrast, when targeting the coding strand, KRAB can be effective....
Long noncoding RNAs (lncRNAs) are linked to cancer via pathogenic changes in their expression levels. Yet, it remains unclear whether lncRNAs can also impact tumour cell fitness via function-altering somatic “driver” mutations. To search for such drive
Double-strand break (DSB)In vitro transcriptionPlasmid DNADNA damage response (DDR) is essential for the maintenance of genomic integrity. We have recently discovered the generation of noncoding RNA from a DNA double-strand break (DSB) in an MRE11-RAD50-NBS1 complex-dependent manner, which ...
The simplest are antisense RNAs transcribed from the opposite DNA strand as the mRNA that they regulate. In some, but not all cases, binding of the sRNA to its mRNA target requires the RNA chaperone Hfq (Møller et al., 2002, Soper and Woodson, 2008, Zhang et al., 2002). Thus, in...
Bats possess a uniquely adapted immune system that enables them to live with viral infections without the expected maladies. The molecular basis and regulation of bats’ immune response is still not fully understood. Long non-coding RNAs (lncRNAs) repres
double-stranded duplex) are incorporated into the AGO proteins, forming the RNA-induced silencing complex (RISC). In the RISC, the miRNA duplex is unwound by helicase, and one strand (the “passenger” strand) of this duplex is degraded. The remaining strand (the “guide” strand) then bind...
During the integration step of the retroviral life cycle, double-strand breaks must be made in the host genome to insert the proviral DNA (Craigie and Bushman, 2012). Once inserted, host machinery must repair the break in the phosphodiester bonds before damage to the host DNA triggers cell ...
Cancers of the female reproductive system include ovarian, uterine, vaginal, cervical and vulvar cancers, which are termed gynecologic cancer. The emergence of long noncoding RNAs (lncRNAs), which are believed to play a crucial role in several different
KEGG pathway analysis revealed that these RNAs are involved in several biological processes, including inflammation, compound metabolic processes, and DNA double-strand break repair [108]. Therefore, the significance of monitoring lncRNA level in vivo and their potential as therapeutic targets cannot be...