Noncoding SequencesNoncoding sequences do not specify an amino acid sequence or a tRNA or rRNA. About 98 to 99% of the human genome is noncoding. They are generally clustered (e.g., in human chromosome 5q31) in...doi:10.1007/978-1-4020-6754-9_11488George P. Rédei...
Noncoding nonconserved and nonrepetitive (any 100-bp fragment that hit the genome 50 times or more was masked; E ≤ 0.001) nucleotide sequences from the gene space of each CNS-containing gene were pooled and used as the control for the CNSs in that region of gene space. The gene space...
Comparative genomics approaches have identified numerous conserved cis-regulatory sequences near genes in plant genomes. Despite the identification of these conserved noncoding sequences (CNSs), our knowledge of their functional importance and selection
The most highly conserved noncoding sequences identified were on average∼70 bp in length with∼0.15 substitutions per site, which is much lower than the 100% conservation over 200 bp used to define mammalian ultraconserved elements4. Nonetheless, the set of 2,012 most highly conserved noncod...
Institute of Molecular Genetics USSR Academy of SciencesJournal of Biomolecular Structure and DynamicsSprizhitski Yu A, Nechipurenko Yu D, Alexandrov AA, Volk- enstein MV (1988) Statistical analysis of nucleotide runs in coding and noncoding DNA sequences. J Biomol Struc Dyn 6:345-358...
Mortlock DP, Pregizer S. 2012. Identifying functional annotation for noncoding genomic sequences. Curr Protoc Hum Genet. Chapter 1:Unit 1.10.Mortlock DP, Pregizer S: Identifying Functional Annotation for Noncoding Genomic Sequences . In: Current Protocols in Human Genetics. John Wiley & Sons,...
Mortlock DP, Pregizer S. 2012. Identifying functional annotation for noncoding genomic sequences. Curr Protoc Hum Genet. Chapter 1:Unit 1.10.Mortlock DP, Pregizer S: Identifying Functional Annotation for Noncoding Genomic Sequences . In: Current Protocols in Human Genetics. John Wiley & Sons,...
Here, the subject of noncoding sequences that influence smooth muscle cell or endothelial cell phenotype will be summarized as will future directions to further advance our understanding of the increasingly complex molecular circuitry governing normal vascular cell differentiation and how such information ...
These studies completed the knowledge of all the noncoding region sequences of both mRNAs, and it was then possible to calculate their exact size. Human α- and β-globin mRNAs are 575 and 626 nucleotides in length, excluding the poly(A). Furthermore, because the coding and 3′ noncoding...
Amplifications were performed with QuantStudio 7 according to standard protocol (Thermo Fisher), and the relative changes in mRNA expression were calculated using the ΔΔCT method. Gene overexpression and knockdown experiments Both the wild-type and mutated lncRNA spliced sequences were synthesised by...