we observe that deep learning models are superior to Gradient Boosting and also that the inclusion of raw sequences features provides the highest predictive power in the pathogenic variant classification task f
Binding and neutralizing antibody titres were correlated with protective efficacy. These data demonstrate that optimization of non-coding regions can greatly improve the immunogenicity and protective efficacy of a non-modified mRNA SARS-CoV-2 vaccine in non-human primates....
Recommendations for classification of variants in non-coding regionsdoi:10.1016/j.pathol.2024.12.094Nicola WhiffinPathology
Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA from various plant species. The primers worked fo...
First, we used HepG2 PCHi-C data to identify target genes of the CAD/MI SNP carrying regulatory regions. Ideally, each looping in PCHi-C represents an interaction between promoter of a gene and its regulatory region. If a promoter of a gene was found to have a looping interaction with ...
[217]). Indeed, impaired RNA metabolism has been correlated with several neurodegenerative diseases. For example, abnormal repeat expansions in the non-coding regions of disease-related genes induce toxic gain-of-function of RNA in myotonic dystrophy, amyotrophic lateral sclerosis, and frontotemporal ...
Additionally, lncRNAs have been identified at promoter regions of PCG-regulated genes in mouse cells; while their role is not yet clear, it has been suggested that they either promote or interfere with PCG binding at target genes [103, 104]. A further example of a lncRNA mediating chromatin...
several thousand probes targeted miscellaneous cDNAs and ESTs whose coding status was not well characterized at the time this commercial probe set was first produced. To update the annotation of these probes and to clarify whether they targeted protein-coding or noncoding regions, a computational pipe...
(Fig.1a, b; Additional file2: Tables S1 and S2). Oligonucleotide probes were designed to capture RNA transcripts produced from intronic and intergenic regions within 1.5-Mb intervals surrounding known breast cancer GWAS signals at the time of capture design (139/196 signals; Fig.1a; Additional ...
CircRNA intermediates are generated to inverse the order of the 5'- and 3'- regions of permuted tRNAs [108, 109].CircRNAs are involved in antiviral immunity The dsRNA-binding proteins, such as retinoic acid-inducible gene-I (RIG-I), melanoma differentiation-associated gene 5 (MDA5), ...