Annotating functional regions in the non-coding genome involves two complementary analysis techniques: comparative analysis, which involves examining DNA sequences, and functional analysis, which involves exami
Surprisingly, the CCDS protein-coding region of the genome was not the most intolerant functional class. We observed that UCNEs17(highly conserved noncoding regions between human and chicken) are ranked as the most intra-species intolerant class (median gwRVIS: −0.99; Mann–WhitneyUvs. interge...
Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA from various plant species. The primers worked fo...
However, the functional understanding of the CAD loci has been limited by the fact that a majority of GWAS variants are located within non-coding regions with no functional role. High cholesterol and dysregulation of the liver metabolism such as non-alcoholic fatty liver disease confer an ...
(both small and long non-coding RNAs) which are generally transcribed from the genomic regions, once considered to be genetically silent, includingintergenic regions,pseudogenes,repetitive sequencesandtransposable elements. The plantncRNAsare classified into small (18–30 nucleotides (nt), medium (31–...
[217]). Indeed, impaired RNA metabolism has been correlated with several neurodegenerative diseases. For example, abnormal repeat expansions in the non-coding regions of disease-related genes induce toxic gain-of-function of RNA in myotonic dystrophy, amyotrophic lateral sclerosis, and frontotemporal ...
several thousand probes targeted miscellaneous cDNAs and ESTs whose coding status was not well characterized at the time this commercial probe set was first produced. To update the annotation of these probes and to clarify whether they targeted protein-coding or noncoding regions, a computational pipe...
Therefore, accurate sequence-based prediction of chromatin features requires a flexible quantitative model capable of modeling such complex dependencies—and those predictions may then be used to estimate functional effects of noncoding variants.基于序列的,可以考虑到更多复杂的情况,这比单纯的依赖现有的motif...
However, even though these regions do not code for protein, they have other functions and carry necessary regulatory information.They can be classified based on the size of the ncRNA. Small noncoding RNA is usually categorized as being under 200 bp in length, whereas long noncoding RNA is ...
noncoding regions [2,3]. Previous studies have interpreted coding variants based on our understanding of the genetic code and splicing [4]. Many existing computational approaches have been developed for prioritizing these variants, such as SIFT [5] and PolyPhen [6]. Noncoding variants, however,...