==反义lncrna==、asRNAs或ancRNAs最早是在单基因研究中发现的,但近年来的strand tiling[^1]和RNA-seq技术的发展,使其成为真核转录组的一个普遍的全基因组特征[152-154]。这一组包含所谓的自然反义转录本(NATs), NATs又被细分为==顺式NATs (cis-NATs)和反式NATs (trans-NATs)==,前者影响相应的有意义...
crRNAs represented by forward arrows bind to the non-coding strand, whilst those shown as reverse arrows bind the coding strand. Used in combination, the crRNA antiparallel pairs should result in Cas9-mediated double-stranded DNA breaks on either side of the targeted region. A similar method ...
We again observed significantly greater effects for sgRNAs targeting the coding strand within the gene body in the FADS1 and FADS2 HCR–FlowFISH CRISPRi tiling screens (P < 1 × 10–15; Fig. 6b,c). These coding strand effects were uniform throughout the transcribed gene body and...
sense strand是指编码链 不作为转录 的另一条DNA链为编码链,又称有意义链. antisense strand是指反义链 在基因的DNA双链中,转录时作为mRNA合成模板的那条 DNA单链叫做模板链或反义链. 还有的两个就不知道了. 分析总结。 antisensestrand是指反义链在基因的dna双链中转录时作为mrna合成模板的那条dna单链叫做...
Here, we wanted to analyze experimentally the incidence of antisense transcription in human cells and to estimate their influence on protein coding expression patterns obtained by double-stranded microarrays. Therefore, we profiled transcription of sense and antisense independently by using strand-specific...
The strand attaches to Argonaute 2 (AGO2), which is the major protein of the RNA-induced silencing complex (RISC). miRNAs are included in the RISC. They can regulate gene expression in a posttranscriptional manner through formation of miRNA response elements (MREs) by binding to specific ...
potassium voltage-gated channel, KQT-like subfamily, member 1 opposite strand 1 LC3B-II/MAP1LC3B microtubule-associated protein 1 light chain 3 beta-II LincRNA long intervening noncoding RNA LINCMD1 long noncoding RNA, muscle differentiation MALAT1 metastasis associated lung adenocarcinoma transcript ...
In hESC lines, methylated non-CG sites are strongly conserved especially within the motif 5’-TAmCAG-3’ on the non-coding DNA strand at 3’ splice junctions[2]. While both hESCs and bees have non-CG modifications at splice junctions, bees differ from hESCs in several respects. In hESCs...
Whiteside EJ, Seim I, Pauli JP, O'Keeffe AJ, Thomas PB, Carter SL, Walpole CM, Fung JN, Josh P, Herington AC, Chopin LK (2013) Identification of a long non-coding RNA gene, growth hormone secretagogue receptor opposite strand, which stimulates cell migration in non-small cell lung canc...
It is important to consider the biology of cancer stem cells with a particular focus on their phenotypic and metabolic plasticity, the most important signaling pathways, and non-coding RNAs (ncRNAs) regulating these cellular entities. Furthermore, the current status of therapeutic approaches against ...