insights into function. Here we use highly multiplexed Tandem-Mass-Tag (TMT)-based mass spectrometry with selective cell surface biotinylation to characterise the classical monocyte surface proteome, then interrogate the phenotypic differences between each monocyte subset to identify novel protein markers. ...
Non-classical Ly6Clo monocytes also initially decreased (by five-fold), but then increased by five-fold relative to day 0 (Fig. 1b) and were the most abundant type of monocyte in blood for the duration of the study (Supplemental Figure S1b). Ly6Cint monocytes were similarly decreased...
Non-classical monocyte homing to the gut via α4β7 integrin mediates macrophage-dependent intestinal wound healingNon-classical monocyte homing to the gut via α4β7 integrin mediates macrophage-dependent intestinal wound healinggut immunologyulcerative...
Finally, PD-L1 expression on these intestinal host non-classical monocyte derived macrophages but not on stroma or other hematopoietic cells regulated alloreactive T cell responses in the intestinal tract. Based on these findings we postulate that a specialized and persistent subpopulation of host non-...
macrophages only and not astrocytes, which is problematic because this is based on limited donors, limited regions of the brain, and most critically astrocytes are heterogenous and they were defined by GFAP expression only and did not include other markers such as S100β. Indeed, a caveat that ...
In addition, a role of miR-181a in the regulation of mitogen-activated protein kinase/extracellular signal-regulated kinase signaling in monocyte-derived dendritic cells (DCs) has been suggested, and sustained expression of miR-181a in DCs may induce higher levels of cluster of differentiation ...
“monocyte counts” in monocytes, “lymphocyte counts” in T cells, and “neutrophil percentage of white cells” for neutrophils (Additional file1: Fig. S4.3a), further underlining the cell type specificity of cmQTLs. To assess how cmQTLs can assist in understanding regulatory logic at disease...
THP-1 cells, a human monocyte cell line, were obtained and verified by ATCC. This cell line (ATCC, TIB-202) was established from a make who had acture monocytic leukemia. Method details CASCADE method The CASCADE method is used to profile the impact of non-coding DNA variants on TF-COF...
“classical” CD16−monocytes are proliferative while CD16+monocytes are anti-proliferative21,22. Additionally, the existence of a “proliferative monocyte” population has been observedin vitro23. Importantly, a recent study has shown that human monocytes can circulate in the blood for as long ...
while other inflammatory markers such as CRP, IL-6, and Lactate Dehydrogenase (LDH) were elevated in the pneumonic group as expected (Fig.1e). Further differentiation of leukocyte subsets revealed lower lymphocyte counts in the pneumonic group, but similar neutrophil and monocyte counts as reported...