【摘要】目的:检测NKp46-Cre介导的黄色荧光蛋白(YFP)报告基因系统在小鼠体内自然杀伤细胞的效率以及特异性.方法:通过ROSA26R-YFP与NKp46-Cre小鼠杂交产生后代并且利用基因型鉴定的方法筛选出双阳性的基因型小鼠,流式细胞术检测小鼠体内免疫器官淋巴结、脾脏以及骨髓中的YFP的表达效率,然后用细胞外表抗体标记脾脏的淋巴细...
鉴于NK细胞依赖于Eomes,而ILC1和NKp46+ILC3依赖于GATA3。作者分别在Ncr1cre/+;Eomesfl/fl小鼠观察到CD49b+细胞显著降低,而CD49b+CD45i.v.-细胞不受影响,在Ncr1cre/+;GATA3fl/fl小鼠观察到CD49b+NK细胞比例不变,而CD49b+CD45i.v.-细胞显著降低。与此同时,作者在人肾炎组织中同样观察到NKp46+ILC1...
鉴于NK细胞依赖于Eomes,而ILC1和NKp46+ILC3依赖于GATA3。作者分别在Ncr1cre/+;Eomesfl/fl小鼠观察到CD49b+细胞显著降低,而CD49b+CD45i.v.-细胞不受影响,在Ncr1cre/+;GATA3fl/fl小鼠观察到CD49b+NK细胞比例不变,而CD49b+CD45i.v.-细胞显著降低。与此同时,作者在人肾炎组织中同样观察到NKp46+ILC1...
cre鼠就是我们常用的带有不同的cre酶的工具鼠统称,可用于繁育交配,比如说B6.129P2-Lyz2 tm1(cre)lfo/NJU, 这种就是我们的带有骨髓细胞特异表达cre酶的模型,还有很多很多在不同组织表达的酶的模型,可以根据自己的实验需求来安排。 Ncr1-cre(Nkp46-cre)小鼠介绍 NK细胞特异表达cre,与带有两个FloxP位点的FloxP小...
目的:检测NKp46-Cre介导的黄色荧光蛋白(YFP)报告基因系统在小鼠体内自然杀伤细胞的效率以及特异性.方法:通过ROSA26R-YFP与NKp46-Cre小鼠杂交产生后代并且利用基因型鉴定的方法筛选出双阳性的基因型小鼠,流式细胞术检测小鼠体内免疫器官淋巴结,脾脏以及骨髓中的YFP的表达效率,然后用细胞表面抗体标记脾脏的淋巴细胞,分析淋...
Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 iCreTbx21 fl/fl mice. "Published figure using CD335 (NKp46) monoclonal antibody (Product # 61-3351-82) in Flow Cytometry" 4.Cellular and molecular immunology ...
Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 iCreTbx21 fl/fl mice. 2.Nature communications Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis. "Published figure using CD335 (NKp46) monoclonal antibody (Product...
Conventional NK cells and ILC1 are partially ablated in the livers of Ncr1 iCreTbx21 fl/fl mice. 2.Nature communications Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis. "Published figure using CD335 (NKp46) monoclonal antibody (Product...
Cx3cr1creErt2R26tdTomand CD45.2i.v. tracking experiments and all experiments for manuscript revisions. A. Taranko, V. Jafari, T.K., A.K., L.F., M.P. and G.M.G. assisted with experiments. F.S. (IF, confocal microscopy, ELISAs, qPCRs), T.L.R., V. Jelencic and S.J....
K.S. performed the Sle1Yaa;Trem2−/−, Ncr1Gfp/Gfp imiquimod, anti-CSF2, NKp46 stimulation, Csf2-tdTomato, Cx3cr1creErt2R26tdTom and CD45.2i.v. tracking experiments and all experiments for manuscript revisions. A. Taranko, V. Jafari, T.K., A.K., L.F., M.P. and G....