Today, three different types of NK cell memory have been described: (i) cytokine-induced memory-like cells, (ii) MCMV-induced memory, and (iii) liver-restricted NK cell memory (Fig. 5.4). Sign in to download full-size image Figure 5.4. Three forms of NK cell memory. (left panel) NK...
Human decidual NK cells form immature activating synapses and are not cytotoxic. Proc Natl Acad Sci U S A. 2005;102:15563–8. CentralKopcow HD, Allan DS, Chen X, Rybalov B, Andzelm MM, Ge B, et al. Human decidual NK cells form immature activating synapses and are not cytotoxic. ...
Full size table Clinical findings suggest that two parameters are critical for adoptively transferred NK cells to mediate therapeutically relevant effects in patients with solid tumors: (1) intratumoral accumulation [34], and (2) persistence in an activated state [28]. Here, we review metabolic an...
Here we demonstrate that CD127−CD103+Eomes+NK cells are the major ILC population in infant intestines during the first months of life, and that their absolute numbers decrease with age. Intestinal CD127+ILCs are also present early in life, but to a lesser extent than NK cells. Infant in...
Full size image NK cells conjugate via CD56- and CD62L-enriched structures NK cells migrating on developmentally supportive stroma adopted a broad leading edge and a uropod-like trailing end. Surprisingly, however, when CD56brightNK cells were found in arrest, they were conjugated to stromal cells...
regimens is asparaginase, which induces apoptosis of NK-cells in vitro [49]. Asparaginase also showed single-agent activity in relapsed/refractory NK/T-cell lymphoma [2,7]. Practically every effective regimen currently used in NK/T-cell lymphoma contains asparaginase or its pegylated form (Table2...
Full size image How the TME can affect NK cell metabolism Changes in cellular metabolism are integrally linked to the functional fate of immune cells [28, 29]. Limiting glycolysis or OXPHOS in human or murine NK cells results in impaired IFN-γ and Granzyme B production and cytotoxicity [15,...
By this process, only NK cells expressing inhibitory receptors for self HLA class I molecules acquire full functional potential, while the “autoreactive” NK cells acquire a state of hyporesponsiveness. The education process is necessary for ensuring self-tolerance by NK cells because the array of...
Full size image CAR design for NK cells The original idea of CARs was designed for T cells in order to improve their strength and functionality toward the target cells [31]. These engineered proteins consist of three essential components: an extracellular domain, a transmembrane region, and an ...
Full size image To assess the efficacy of GPC3-targeting CAR-NK cells for HCC cells, a CAR construct with anti-GPC3 scFv sequence was generated (Fig. 1B), based on previously reported CAR backbone containing CD8α hinge, NKG2D transmembrane domain, 2B4 co-stimulatory domain and CD3ζ activati...