Several NK cell sources have been used for adoptive transfer. The NK-92 cell line has been approved for clinical application by the Food and Drug Administration (FDA) [6]. Although this cell line displays cytotoxicity against tumor cells, it has poor ADCC potential because it lacks the CD16 ...
The expression of certain genetic patterns in NK cells or tumor cells can affect brain tumor recurrence and aggressiveness. Silencing ofKLRC3in NK cells, decreases the self-renewal capacity, invasion, proliferation, radio-resistance, and tumorigenicity of the U87-MG glioma cell line52. CRISPR-Cas9-...
After 48 h, the cells were lysed, and the firefly and Renilla luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega). Each fragment containing the putative miRNA-binding sites was cloned in psiCHECK-2 immediately downstream of the gene encoding renilla ...