At the end of the culture period, cells were rinsed twice with PBS and lysed with 1× lysis buffer (20 µL/well). Luciferase activity was measured using a luciferase assay system according to the Acta Pharmacologica Sinica www.chinaphar.com Zeng XZ et al npg 257 manufacturer's ...
At the end of the culture period, cells were rinsed twice with PBS and lysed with 1× lysis buffer (20 μL/well). Luciferase activity was measured using a luciferase assay system according to the manufacturer's instructions (Promega, Madison, WI, USA)14,16. Real-time PCR analysis...
Cells were then washed with PBS and stained with 2 μM JC1 for 30 min. Samples were finally washed with PBS and examined under confocal microscope. Release of cytochrome c from mitochondria was assessed by confocal microscopy. Briefly, cells were washed with PBS after incubation with α-sola...
Then the cells were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature. After the removal of the fixative, A549 cells were washed with PBS and permeabilized with 0.3% Triton X-100 for another 15 min, the cells were incubated with 100 μL 0.3% hydrogen peroxide solution ...
*P < 0.05 compared with the empty group (PBS treated cells). 3.4. Expressions of GAS5 in PCa cells GAS5 has been reported as a key indicator for the diagnosis and prognosis prediction of PCa [27,28]. However, the expression level of GAS5 was controversial in PCa [29,30]. In this ...
Cells were harvested using a cell lifter and washed twice with phosphate buffer saline (PBS). LC–MS/MS analysis was performed as a service by Bioproximity LLC (Chantilly, VA, USA). Pooled genome-wide shRNA library screen MCF-7 cells were transduced with MISSION LentiPlex Human Pooled sh...
The next day, the primary antibody was removed, and the membranes were washed in PBS, and incubated with appropriate secondary antibody (1:100) for 2 h at room temperature, followed by enhanced chemiluminescence (ECL). β-ac-tin was selected as an internal control to compare protein ...
(St. Louis, MO, USA). The absolute ethanol and N,N-Dimethylformamide (DMF) came from Merk (Darmstadt, Germany). Venlafaxine, epinine and all other reagents were of an analytical grade (from Sigma-Aldrich). Orthophosphoric acid was used to prepare the phosphate buffer solution (0.1 M, PBS...
The cells were scraped using a cell scraper and centrifuged at 10.000 rpm for 10 min, after which the sample was rinsed again with PBS. Proteins were isolated with RIPA buffer. After cell lysates sonication, Nutrients 2018, 10, 672 5 of 19 total protein contents were determined by using a...
toxins Article Fraternine, a Novel Wasp Peptide, Protects against Motor Impairments in 6-OHDA Model of Parkinsonism Andréia Mayer Biolchi 1, Danilo Gustavo Rodrigues de Oliveira 1,*, Henrique de Oliveira Amaral 1, Gabriel Avohay Alves Campos 1, Jacqueline Coimbra Gonçalves 1, Adolfo Carlos ...