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Cells were harvested using a cell lifter and washed twice with phosphate buffer saline (PBS). LC–MS/MS analysis was performed as a service by Bioproximity LLC (Chantilly, VA, USA). Pooled genome-wide shRNA library screen MCF-7 cells were transduced with MISSION LentiPlex Human Pooled sh...
When the cells grew to form visible colonies, the supernatants were discarded and the colonies were fixed with 4% paraformaldehyde for 30 min, and then washed with PBS and stained with crystal violet for 15 min. For soft agar colony formation assay, PC9GR cells suspended in complete culture ...
Cells were then washed with PBS and stained with 2 μM JC1 for 30 min. Samples were finally washed with PBS and examined under confocal microscope. Release of cytochrome c from mitochondria was assessed by confocal microscopy. Briefly, cells were washed with PBS after incubation with α-sola...
The blank group was treated with PBS and X-ray irradiation insult or not, and the control group was treated with 1‰ DMSO with X-ray irradiation insult or not. The survival fractions = the number of colonies/ (number of inoculated cells × plating efficiency), and the radiation ...
After treatment, cells were fixed with 3.7% formaldehyde in PBS for 10 min at room temperature and nuclei were counterstained with 1 µg/mL DAPI for 5 min before mounting. Cells were imaged with an Olympus BX-50 upright microscope and the mean fluorescence intensity of nuclear and ...
DLD-1 cells were sub-cultured every 3–4 days by their rinsing with phosphate buffered saline (PBS), adding trypsin to detach the cells from the tissue culture flask, and transferring 10–20% of the harvested cells to a new flask containing fresh growth media. 3.5.5. Cytotoxicity against ...