NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated in 1962 at the New York University School of Medicine Department of Pathology. 3T3 refers to the cell transfer and inoculation protocol for the line, and means “3-day transfer, inoculum 3 x 105 cells.” Using...
转染NIH3T3 细胞 按照以下步骤将质粒 DNA 转染至24孔板中的 NIH3T3 细胞中(对于其他规格,请参见下文的扩大或缩小转染规模)。指定的所有数量和体积均以每个孔为基础。 转染前一天,用胰蛋白酶处理细胞并进行计数。在含有 0.5 mL 完全生长培养基的每个孔中...
NIH/3T3 cell culture medium 한글명:NIH/3T3 cell culture medium 카스 번호 상품명:NIH/3T3 cell culture medium CBNumberCB49404375 포뮬러 무게0 MOL 파일Mol file NIH/3T3 cell culture medium 공급 업체
cell culturefibroblastsnanotechnologyTraditional flat tissue cell culture dishes have consisted of polystyrene treated with plasma gases for growing, subculturing, and studying cell behavior in vitro. However, increasingly it has been observed that mimicking natural tissue properties (such as...
1. Cell culture The mouse NIH3T3 fibroblast cell line was maintained in DMEM high glucose medium (Invitrogen) containing 10% calf serum (Sigma) and 100 U ml−1 penicillin/streptomycin (Invitrogen). Human 293T cells were maintained in DMEM high glucose medium containing 10% fetal calf serum ...
(L) and width (W) of the tumors and the formula (L × W2)/2 [33]. The tumorigenicity assays were terminated by sacrificing the mice at 5 weeks (for HeLa cells) or 7 weeks (for NIT3T3 cells) after inoculation. All animal studies were conducted under an approved protocol by the ...
Constitutive activation of RhoA-dependent RhoA kinase (ROCK) signalling is known to promote cellular transformation and the ROCK inhibitor Y-27632 has the ability to suppress focus formation of RhoA transformed NIH3T3 cells. Sixty-four novel structural a
High density lipoproteins (HDLs) play a crucial role in removing excess cholesterol from peripheral tissues. Although their concentration is lower during conditions of high cell growth rate (cancer and infections), their involvement during cell prolifera
To evaluate the effects of EEACP on the intracellular signaling pathways activated by UVB irradiation, the phosphorylation status of AKT and MAPK in NIH-3T3 cells was assessed using Western blotting, following a previously described protocol [19]. Briefly, after cell lysis in lysis buffer [20] ...
Cell Culture and RNA Isolation Murine NIH 3T3 cells were cultured in DMEM containing 2% FCS and 8% CS in a water-saturated 7% CO2 incubator. Serum stimulation of quiescent, serum-starved NIH 3T3 cells was performed as described previously (29). Murine RAW 264.7 cells were cultured in DMEM ...