Volume-activated chloride channels contribute to lipopolysaccharide plus nigericin–induced pyroptosis in bone marrow–derived macrophages - ScienceDirectXiaomin Ye aXiaoyong Liu aWenjun Wei aHuiping Yu aXiaobao Jin aJinwei Yu aChunmei Li aBin Xu aXinmin Guo bJianwen Mao a
In a lipopolysaccharide (LPS)-stimulated and nigericin-treated human monocytic cell line (THP-1), apoptosis was induced, as assessed as to a decrease in cell size, chromatin condensation, after exposure of phosphatidylserine and DNA fragmentation [19]. Infection with human cytomegalovirus (HCMV) ...
Notably, we found that nigericin-induced pyroptosis promoted the infiltration and activation of T cells, as well as showing a synergistic therapeutic effect when combined with anti-PD-1 antibody treatment. This study provides a potential strategy to utilize nigericin to boost the...
Pyroptosis-related cytokines were evaluated using RT-PCR for interleukin-1尾 (IL-1尾)and interleukin- 18 (IL18). Non-cytotoxic concentration of Psoralidin (5.0M) significantly inhibited LPS/Nigericin induced inflamasome activation. In addition, pretreatment with psoralidin suppr...
MRS2211 inhibited the release of LDH induced by gp120, whereas nigericin aggravated the damage. The combination of MRS2211 and nigericin can eliminate the inhibition of MRS2211 on pyroptosis and counteract the activation of NLRP3 by nigericin. All data were expressed as mean ± SEM (n = 3/...
Inflammasomesare cytosolicmultiprotein complexesthat are responsible for innate immunity, eventually resulting in cell death calledpyroptosis[19]. Among the variousinflammasomes, NOD-like receptorpyrindomain-containing 3 (NLRP3) is the most well-characterized which gets activated in conditions oftissue damag...
Yaron, J. R. et al. The oxindole Syk inhibitor OXSI-2 blocks nigericin-induced inflammasome signaling and pyroptosis independent of potassium efflux. Biochemical and biophysical research communications 472, 545-550, doi:10.1016/j.bbrc.2016.03.021 (2016)....
(4) Finally, inflammasome formation leads to the processing and activation of caspase-1, which cleaves the pro-forms of IL-1β and IL-18, resulting in the release of the mature cytokines and inducing cell death by pyroptosis.Figure adapted from Haneklaus et al., 2013.44 The NLRP3 ...