Moreover, the concentration of purified eGFP by PDA/Fe3+/Ni2+ was reported 138.83 mu g/ml by the fluorescent signals, which is almost equal to or more than the protein purified by commercial Ni-NTA column (108.28 mu g/ ml). The stability of PDA/Fe3+/Ni2+ has also been evaluated ...
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1) 将NTA树脂装入合适的层析柱,层析用10倍NTA体积的NTA-0 Buffer洗。 2)将样品(步骤2(6))加到NTA层析柱中,流速控制在15ml/小时左右,收集穿透部分,用于SDS/PAGE分析蛋白质的结合情况。 3)层析用5倍NTA体积的NTA-0 Buffer洗,流速控制在30ml/小时左右。 4)分别用5倍NTA体积的NTA-20,NTA-40,NTA-60,NTA-...
Product Name Index NI-424 (IC) Applications
Purification and on-column refolding of His-tagged PhaZ depolymerase from Pseudomonas putida KT2442 on Ni2+NTA agarose gel: preparation of a novel immobilized biocatalystPichia pastorisFed-batch cultivationFormaldehyde dehydrogenase promoterRhizopus oryzae lipase...
The purification of the iNOS/CaM complex from 4L BL21 (DE3) culture had been performed on 2', 5' 鈥揂DP Sepharose, NiNTA, and S200 gel filtration column. The purified active iNOS had been collected from only NiNTA column with 89% total iNOS yield and 200-fold purification. However, ...
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