Affinity purification of HC-Pro of potyviruses with Ni2+-NTA resin. J Virol Methods 76, 19-29.Kadouri, D., Peng, Y., Wang, Y., Singer, S., Huet, H., Raccah, B. and Gal-On, A. (1998) Affinity purification of HC-Pro of potyviruses with Ni2+-NTA resin. J. Virol. Methods,...
aWestern blot assay indicated that purified rE2-a, rE2-b[translate] aE.coli, respectively, by densitometric scanning (Figure[translate] adisplayed a single band with a molecular weight of[translate] awere purified by Ni2+-NTA resin under denaturing conditions[translate]...
L00250-2-High-Affinity Ni-NTA Resin
镍柱琼脂糖树脂(Ni-NTA resin)再生方法摘要: 镍柱纯化是为了纯化带有6个组氨酸(碱性氨基酸)标签的重组蛋白而设计,具有高度的亲和力和选择性。当Ni-NTA琼脂糖的颜色由浅蓝色变成灰褐色或白色时,需彻底再生后才能使用。再生方法总体可分为除去柱上残余蛋白、除镍、挂镍等三个步骤。先用变性剂和乙醇除去残余蛋白和脂类...
Ni-NTA resin and GST resin were purchased from Smart-Lifesciences (Changzhou, China). Ni-NTA Magnetic Agarose Beads were purchased from BEAVER Nano-technologies (Suzhou, China). 96-well nonbinding surface (NBS) microplates were purchased from Corning (New York, USA). Rabbit anti-mouse IgG ...
固化Ni2+层析树脂(如 ProBond,Invitrogen 公司;HisTrap,Pharmacia 公司;His.Bind Resin,Novagen 公司;NTA-Ni2+-琼脂糖,Qiagen 公司) 目前最常用的金属螯合亲和载体是次氨基兰乙酸酯(NTA)-Ni2+-琼脂糖,可从上述公司购买,另外还有不同性质的其他载体。据说一种独特的 Co2+螯合物(Talon) 对 His-6 的亲合力更高...
产品名称：Ni-NTA His Bind Resin 品牌：百奥莱博 产地：北京 价格：面议 点击查看产品详情 联系电话：...
The ferrite materials need to be dispersed in suitable binder resin matrix to realize the practical microwave absorbing products. Therefore, studies on MW absorbing composites with dispersed magnetic ferrite materials in polymer/resin matrix has always been a subject of interest27. Dispersion of ferrite...
Immobilization of GS on the affinity resin Ni2+-NTA followed by on-column modification with diethyl pyrocarbonate affords asymmetrically modified GS that self-assembles only to the extent of 'short' dimeric GS tubes, as demonstrated by electron microscopy, dynamic light scattering and atomic force ...