Ni-NTA matrix Empty columns Wash buffer Elution buffer Wash buffer (1 liter):50 mM NaH 2 PO 4 6.90 g NaH 2 PO 4 ·H 2 O (MW 137.99 g/mol)300 mM NaCl 17.54 g NaCl (MW 58.44 g/mol)20 mM imidazole 1.36 g imidazole (MW 68.08 g/mol)0.05% Tween 20 Adjust pH to 8....
Drager, GKirschning, A
基质(Matrix) 交联的6%琼脂糖凝胶 粒径(Bead size) 45-165 µm 载量(Capacity) >40 mg 6×His-tagged protein/mL 基质 耐压(Tolerance Pressuremax) 0.1 MPa, 1 bar储存缓冲液(Buffer) 含20%乙醇的1×PBS 储存条件2~8℃保存,有效期2年。使用说明...
此外,因其基质的耐压性(可耐受最高0.3 MPa的压力),该产品可以用于工业大规模蛋白的纯化,可在相对较高的流速下,实现对目的蛋白的纯化。 产品性质 基质(Matrix) 高度交联的6%琼脂糖凝胶 粒径(Bead size) 45-165 µm 载量(Capacity) >40 mg 6×His-tagged protein/mL基质...
30% ethanol to inhibit microbial growth. The matrix can be stored for up to one week in any ...
最后需要指出的是QIAGEN的Ni-NTA产品种类齐全,从ng级到生产级别(kg)都有相应的产品提供(见下图),根据手中要纯化的蛋白的数量和特点选择合适的Ni-NTA产品是我们迈向纯化实验成功的第一步。
matrix: with ni2 + highly cross-linked 6% agarose; the average particle size: 90 um; dynamic binding capacity: break through capacity at 10%, to 150 cm / h, about 40 mg histidine-tagged protein / mlfiller;metal ion load: about 15 μmol、70% acetic acid30 min, 30% 2-异丙醇1 h,...
产品介绍 Ni-NTA Agarose is an affinity chromatography matrix for purifying recombinant proteins carrying a His tag. Histidine residues in the His tag bind to the vacant positions in the coordination sphere of the immobilized nickel ions with high specificity and affinity 下单购买...
基质(Matrix) 高度交联的6%琼脂糖凝胶 粒径(Bead size) 45-165μm 载量(Capacity) >40mg 6×His-tagged protein/mL基质 耐压(Tolerance Pressuremax) 0.3MPa, 3bar 储存缓冲液(Buffer) 含20%乙醇的1×PBS 柱子尺寸(Column Size) 1.6×2.5cm(5mL) ...
此外,因其基质的耐压性(可耐受*0.3 MPa的压力),该产品可以用于工业大规模蛋白的纯化,可在相对较高的流速下,实现对目的蛋白的纯化。 产品性质 基质(Matrix) 高度交联的6%琼脂糖凝胶 粒径(Bead size) 45-165 μm 载量(Capacity) >40 mg 6×His-tagged protein/mL基质...