BSA是Bulk Segregation Analysis 的英文首字母缩写,翻译过来就是分离群体分组分析或者是混池分离群体分析。BSA基于遗传分离群体的极端表型构建混池,通过混池中变异的频率差异筛选与表型关联的分子标记,是一种简便高效的性状定位方法。在下一代测序(NGS,next-generation sequencing)的背景下,BSA因其快速的定位和超高的性价...
With the development of next‐generation sequencing (NGS) technology in the past 10 years, BSA methods and technical systems have been gradually developed and improved. At the same time, the ever‐decreasing costs of sequencing accelerate NGS‐based BSA application in different species, including ...
YG, and LL carried out assessment of lint percentage. XW, SL, DF, and HS performed BSA analysis. XW, XZ, YS and XD carried out fine mapping of candidate genes. XW wrote the manuscript. All authors have read and approved the final version of the manuscript. ...
(非必需)牛血清白蛋白(BSA)(50 mg/mL)(例如 Invitrogen™ UltraPure™ BSA 50 mg/mL, AM2616) Hard-Shell® 96-Well PCR Plates, low profile, thin wall, skirted, red/clear (Bio-Rad™, cat # HSP9611) Thermo Scientific™ Abgene™ 96 Well 1.2 ml Polypropylene Deepwell Storage Plate...
QTL-seq is an efficient strategy for QTL identification that takes advantage of bulked- segregant analysis (BSA) and next-generation sequencing (NGS). In the present study, we conducted QTL mapping and QTL-seq of cucumber fruit length. QTL mapping identified 8 QTLs for immature and mature ...
Next Generation Sequencing Bulk Segregant Analysis (NGS-BSA) is efficient in detecting quantitative trait loci (QTL). Despite the popularity of NGS-BSA and the R statistical platform, no R packages are currently available for NGS-BSA. We present QTLseqr, an R package for NGS-BSA that identifi...
BSR是将BSA与RNA-seq结合起来的分析方法,与重测序BSA不同的是,在分离群体中选择极端性状的个体构建两个池,提取两个池的总RNA,进行转录组测序,根据混池个数和物种的基因组大小确定测序的数据量。基于转录组数据同样开发SNP标记,用经典贝叶斯算法找到与突变基因共分离的SNP位点和区间,最后预测候选基因 (Liu et al....
QTL-seq is an efficient strategy for QTL identification that takes advantage of bulked-segregant analysis (BSA) and next-generation sequencing (NGS). In the present study, we conducted QTL mapping and QTL-seq of cucumber fruit length. QTL mapping identified 8 QTLs for immature and mature fruit...
BSR是将BSA与RNA-seq结合起来的分析方法,与重测序BSA不同的是,在分离群体中选择极端性状的个体构建两个池,提取两个池的总RNA,进行转录组测序,根据混池个数和物种的基因组大小确定测序的数据量。基于转录组数据同样开发SNP标记,用经典贝叶斯算法找到与突变基因共分离的SNP位点和区间,最后预测候选基因 (Liu et al....
These microgels were tested with Rhodamine B labeled dextran and BSA protein. The release of the drugs was controlled by pH and correspondingly by the degree of degradation of the nanostructure. Another type of hybrid nanogel containing benzoacetal groups as the degradable linkers was used by Stein...