The nuclear translocation of NFkB in human cells has been extensively studied with the ImageStream technology during the last decade. However, similar studies in murine cells has not been done. In this project, NFkB nuclear translocation in a murine cell line is measured using ImageStream ...
(RHD) of 300 aa length. These RHD domain have multiple functions such as dimerization, DNA binding, interaction with IkBs, and nuclear translocation. Although the NF-kB protein family members can form up to 15 different dimers, but many of them has not yet demonstrated. The most abundant ...
Inhibits translocation of the NF-κB active complex into the nucleus. In mouse endothelial LE-II cells induced with LPS, inhibition of NF-κB nuclear translocation was 88% at 50µg/ml (18µM). In human monocytic THP-1 cells induced by LPS or TNF-αthere was 85% inhibition at 100...
阻断CD40和CD154(也称为“gp39”)之间的相互作用的药物如CD40和/或CD154的特异性抗体,融合蛋白如依那西普、从CD40和/或CD154gp39(例如CD40Ig和CD8gp39)构建的融合蛋白,抑制剂如核转位抑制剂(nuclear translocation inhibitor)、NF
which is followed by its ubiquitination and subsequent degradation. This results in the exposure of the nuclear localization signals (NLS) on NF-kB subunits and the subsequent translocation of the molecule to the nucleus. In the nucleus, NF-kB binds with a consensus sequence (5'GGGACTTTCC-3'...
The ARD mediates interaction with the NF-κB/Rel proteins obstructing their nuclear localization signal (NLS) within the RHD of the Rel proteins and restricting nuclear translocation. Phosphorylation of two N-terminal serine residues (SS) of IκB triggers polyubiquitination and proteasome-mediated ...
NF-kB与中枢神经系统退行性疾病.ＮＦ－κＢ与中枢神经系统退变性疾病 龚云涛 北京大学神经科学研究所 摘要 同时也是细胞凋亡信号通路中一个重要的转录调节因子炎症反应及凋亡的基因转录来发挥作用 近年来尤其是在发 生神经退变的部位可以检测到特异激活的ＮＦ－κＢ 一直争论不休本文旨在回顾近年来ＮＦ－κＢ在神经...
NF-kB与中枢神经系统退行性疾病.ＮＦ－κＢ与中枢神经系统退变性疾病 龚云涛 北京大学神经科学研究所 摘要 同时也是细胞凋亡信号通路中一个重要的转录调节因子炎症反应及凋亡的基因转录来发挥作用 近年来尤其是在发 生神经退变的部位可以检测到特异激活的ＮＦ－κＢ 一直争论不休本文旨在回顾近年来ＮＦ－κＢ在神经...
Inhibition of nuclear translocation of NF-kB specifically blocks TNF-a-induced cell proliferation. The TNF-a also promotes tumour cell survival through the induction of genes encoding NF-kB- dependent antiapoptotic molecules (Shishodia and Aggarwal, 2004). In addition, TNF-a not only acts as an...
Hydrocortisone fails to inhibit LPS induced nuclear NF-κB1 translocation in deletion allele carriers of the NFKB1 promoter polymorphism (-94ins/delATTG). In septic shock, hydrocortisone treatment is associated with markedly increased 30-day-mortality only in such carriers. Accordingly, previous hetero...