报告基因分析系统(reporter gene assay)的建立,给NF- k B的转录活性评价带来了极大方便。(1) 3 一 半乳糖苷酶(3 -galatosidase , 3 -ga1)报告基因:大肠杆菌编码的 3 -gal 可以水解乳糖生成半乳糖, 分光光度计检测颜色改变可反映转 录活性。 3 -gal 基因现常用作转染的参照体系。 20、(2) 荧光素 ...
报告基因分析系统(reporter gene assay)的建立,给NF-κB的转录活性评价带来了极大方便。(1)β一半乳糖苷酶(β-galatosidase,β-ga1)报告基因:大肠杆菌编码的β-gal可以水解乳糖生成半乳糖,分光光度计检测颜色改变可反映转录活性。β-gal基因现常用作转染的参照体系。(2)荧光素(1uciferase,luc)报告基因:luc能...
Luciferase Reporter Gene Assay Kit 萤火虫荧光素酶报告基因检测试剂盒 11401ES60/76/80 100 T/500 T/1000 T pGM-CMV Luciferase Reporter Plasmid Positive Control pGM-CMV-Luc荧光素酶报告基因质粒阳性对照 11556ES03 1 μg Luciferase Reporter Plasmid negative control (荧光素酶报告基因质粒阴性对照)...
The NF-kB/SEAP is a reporter gene assay for the detection of NF-kB activation and contains a cell line modified for this purpose. This cell line is derived from HEK 293 (Human Embryonal Kidney) cells. 产品介绍 NF-kB/SEAP Stable Reporter Cell Line Description(产品描述) The NF-kB/SEAP ...
报告基因分析系统(reporter gene assay)的建立,给NF-κB的转录活性评价带来了极大方便。(1) β一半乳糖苷酶(β-galatosidase,β-ga1)报告基因:大肠杆菌编码的β-gal可以水解乳糖生成半乳糖,分光光度计检测颜色改变可反映转录活性。β-gal基因现常用作转染的参照体系。(2)荧光素(1uciferase,luc)报告基因:luc能...
(E) NF-kB activity was detected by the luciferase reporter gene assay. **P < 0.01, n = 3. Full size image We further examined the effects of ethanol on the transcription of NF-κB using a luciferase reporter. As shown in Figure 5E, ethanol increased NF-κB transcriptional ...
Comparative evaluation of different eultivars of Flos Chrysanthemi by an anti-inflammato- ry-based NF-KB reporter gene assay coupled to UPLC-Q/TOF MS with PCA and ANN [J]. J Ethnopharmacol, 2015, 174:387.Han Y Q, Zhou M G, Wang L Q, et al. Comparative evaluation of different ...
NF-κB家族系列病毒产品&研究工具 汉恒生物—NF-κB家族系列病毒产品&研究工具 研究背景 核因子活化B细胞κ轻链增强子（nuclear factor kappa-light-chain-enhancer of activated B cells，简称为NF-κB）是一种控制DNA转录的蛋白复合体。NF-κB几乎存在于所有类型的动物细胞中并参与细胞对诸多刺激的响应，这些刺激包括...
Regulation of PLPP3 gene expression by NF-kB family transcription factors. J Biol Chem. 2019;294(38):14009–19. Article PubMed PubMed Central Google Scholar Wu ZH, Wong ET, Shi Y, Niu J, Chen Z, Miyamoto S, Tergaonkar V. ATM- and NEMO-dependent ELKS ubiquitination coordinates TAK1-...
Cells were lysed using 50 μL (for 96-well plate) of reporter lysis buffer from Promega by incubating for 5 min on a shaker, and stored at −80 °C. The luciferase assay was performed using the Luc assay system from Promega. The gene product, luciferase enzyme, reacts with ...